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Analytical characterization of mannosylerythritol lipid biosurfactants produced by biosynthesis based on feedstock sources from the agrofood industry

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Abstract

Mannosylerythritol lipids (MELs) are currently one of the most promising biosurfactants because of their multifunctional applications and good biodegradability. Depending on the yeast strain and the feedstock used for the fermentation process, structural variations in the MELs obtained occur. Therefore, MELs produced by Pseudozyma aphidis DSMZ 70725 with a soybean oil feedstock were characterized by chromatography and mass spectrometry (MS). Column chromatography with silica provided fractionation of the different types of MEL. High-performance liquid chromatography combined with MS was employed for the analysis of the MEL fractions and crude mixtures. A characteristic MS pattern for the MELs was obtained and indications of the presence of new MEL homologues, showing the incorporation of longer and more unsaturated fatty acid chains than previously reported, were given. Gas chromatography–MS analysis confirmed the presence of such unsaturated fatty acid chains in the MELs, demonstrating the incorporation of fatty acids with lengths ranging from C8 to C14 and with up to two unsaturations per chain. The incorporation of C16 and C18 fatty acid chains requires further investigation. MS/MS data allowed the unambiguous identification of the fatty acids present in the MELs. The product ion spectra also revealed the presence of a new isomeric class of MELs, bearing an acetyl group on the erythritol moiety.

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Acknowledgements

M.O. is grateful to the team at the University of Valencia for allowing him to spend some months in their laboratory. T.G. and A.C. acknowledge Research Foundation Flanders (FWO) for financial support. The authors would like to thank Dionex for providing the Acclaim surfactant column and Waters for the provision of the hydrophilic interaction liquid chromatography column. This study was financed by the University of Antwerp (KARAMEL UA-BOF project). Filip Lemière thanks the Hercules foundation for financial support in purchasing the MS instrumentation.

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Authors and Affiliations

  1. Department of Industrial Sciences and Technology, Karel de Grote University College, Research Group Industrial Biotechnology, Salesianenlaan 30, 2660, Hoboken, Antwerp, Belgium

    Matthias Onghena, Eliane Goossens & Marc Wijnants

  2. Toxicological Centre, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610, Wilrijk, Antwerp, Belgium

    Tinne Geens, Hugo Neels & Adrian Covaci

  3. Department of Pharmacy, University of Valencia, Avenida Vicent Andres Estelles s/n, 46100, Burjassot, Valencia, Spain

    Yolanda Pico

  4. Nucleoside Research and Mass Spectrometry Unit, Department of Chemistry & CeProMa, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium

    Filip Lemiere

Authors
  1. Matthias Onghena
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  2. Tinne Geens
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  3. Eliane Goossens
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  4. Marc Wijnants
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  5. Yolanda Pico
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  6. Hugo Neels
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  7. Adrian Covaci
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  8. Filip Lemiere
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Correspondence to Adrian Covaci.

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Footnote: Published in the special issue Advances in Analytical Separations with Guest Editors Yolanda Pico and Joan O. Grimalt.

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Onghena, M., Geens, T., Goossens, E. et al. Analytical characterization of mannosylerythritol lipid biosurfactants produced by biosynthesis based on feedstock sources from the agrofood industry. Anal Bioanal Chem 400, 1263–1275 (2011). https://doi.org/10.1007/s00216-011-4741-9

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  • DOI: https://doi.org/10.1007/s00216-011-4741-9

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