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Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion

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Abstract

A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-μm-wide channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open headstage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups.

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Acknowledgements

Financial support through EU grant MRTN-CT-2005–019335 (Translocation) is acknowledged. C.F. is a fellow of the International Center for Transdisciplinary Studies (ICTS) at Jacobs University Bremen gGmbH.

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Authors and Affiliations

  1. Jacobs University Bremen, Campus Ring 1, 28759, Bremen, Germany

    Tivadar Mach, Catalin Chimerel, Jürgen Fritz & Mathias Winterhalter

  2. Nanion Technologies GmbH, Erzgiessereistr. 4, 80335, Munich, Germany

    Niels Fertig

  3. International Center for Transdisciplinary Studies, Jacobs University Bremen, Campus Ring 1, 28759, Bremen, Germany

    Claus Fütterer

  4. INB-1, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany

    Claus Fütterer

Authors
  1. Tivadar Mach
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  2. Catalin Chimerel
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  3. Jürgen Fritz
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  4. Niels Fertig
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  5. Mathias Winterhalter
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  6. Claus Fütterer
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Corresponding author

Correspondence to Claus Fütterer.

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Mach, T., Chimerel, C., Fritz, J. et al. Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion. Anal Bioanal Chem 390, 841–846 (2008). https://doi.org/10.1007/s00216-007-1647-7

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  • DOI: https://doi.org/10.1007/s00216-007-1647-7

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