Skip to main content
SpringerLink
Log in

Determination of sub-ppb reserpine by an optosensing device based on photochemically induced fluorescence

  • Original Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

A flow injection–solid-phase spectroscopy (FI-SPS) system implemented with photochemically induced fluorescence (PIF) is described for the rapid and very sensitive determination of reserpine in biological fluids and pharmaceutical formulations. An intensively fluorescent photoproduct is in-line generated, retained on C18 silica gel in the detection area and monitored at 394/489 nm (λ ex/λ em). After the establishment of the appropriate working variables, the system is calibrated at two different injection volumes, 100 and 800 μL, achieving detection limits of 0.33 and 0.05 ng mL−1, respectively. The RSD for reserpine at 2 ng mL−1 (800 μL) was 1.5% (n = 10). The sampling rates were 46 and 43 h−1 for each injection volume, respectively. The potential interference of some common species coexisting with reserpine in the analysed samples was also studied. The procedure was successfully applied to commercial formulations, urine and serum without any previous treatment of samples. Recoveries ranged from 94.9 to 100.2%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Singh DK, Srivastava B, Sahu A (2004) Anal Sci 20:571–573

    Article  CAS  Google Scholar 

  2. Varma SR, Martínez Calatayud J, Mottola HA (1990) Anal Chim Acta 233:235–241

    Article  CAS  Google Scholar 

  3. Zhang H, Wu K (2005) Microchim Acta 149:73–78

    Article  CAS  Google Scholar 

  4. Zhang F, Li H (2005) Electrophoresis 26:1692–1702

    Article  CAS  Google Scholar 

  5. Cao W, Yang X, Wang E (2004) Electroanal 16:169–174

    Article  CAS  Google Scholar 

  6. Pinotsis N, Calokerinos AC, Baeyens WRG (2000) Analyst 125:1307–1311

    Article  CAS  Google Scholar 

  7. Song ZH, Zhang N (2003) Anal Lett 36:41–57

    Article  CAS  Google Scholar 

  8. Srivastava A, Tripathi AK, Pandey R, Verma RK, Gupta MM (2006) J Chromatogr Sci 44:557–560

    CAS  Google Scholar 

  9. Cieri UR (1998) J AOAC Int 81:373–380

    CAS  Google Scholar 

  10. Anderson MA, Wachs T, Henion JD (1997) J Mass Spectrom 32:152–158

    Article  CAS  Google Scholar 

  11. Fialkov AB, Gordin A, Amirav A (2003) J Chromatogr A 991:217–240

    Article  CAS  Google Scholar 

  12. Settimj G, Di Simone L, Del Giudice MR (1976) J Chromatogr 116:263–270

    Article  CAS  Google Scholar 

  13. Aly FA, El-Brashy A, Belal F (1994) Anal Chim Acta 291:141–145

    Article  CAS  Google Scholar 

  14. Walash MI, Belal F, Aly FA (1998) Talanta 35:731–733

    Article  Google Scholar 

  15. Smith WM (1977) J AOAC Int 60:1018–1021

    CAS  Google Scholar 

  16. Haycock RP, Sheth PB, Connolly RJ, Mader WJ (1966) J Agric Food Chem 14:437–440

    Article  CAS  Google Scholar 

  17. Mahedero MC, Aaron JJ (1992) Anal Chim Acta 269:193–198

    Article  CAS  Google Scholar 

  18. Matthews CZ, Woolf EJ, Mazenko RS, Haddix-Wiener H, Chavez-Eng CM, Constanzer ML, Doss GA, Matuszewski BK (2002) J Pharm Biomed Anal 28:925–934

    Article  CAS  Google Scholar 

  19. Martínez Calatayud J, Gómez Benito C (1991) Anal Chim Acta 245:101–107

    Article  Google Scholar 

  20. Xu JG, Chen HP, Guo XQ, Zhao YB (1995) Anal Chim Acta 302:207–214

    Article  CAS  Google Scholar 

  21. Chen H, He Q (2000) Talanta 53:463–469

    Article  CAS  Google Scholar 

  22. Molina Díaz A, Fernández de Córdova ML, Ruiz Medina A (2002) J Pharm Biomed Anal 28:399–419

    Article  Google Scholar 

  23. Ortega Barrales P, Fernández de Córdova ML, Molina Díaz A (1998) Anal Chim Acta 376:227–233

    Article  CAS  Google Scholar 

  24. López-Flores J, Fernández-de Córdova ML, Molina-Díaz A (2005) Anal Chim Acta 535:161–168

    Article  Google Scholar 

  25. IUPAC (1978) Spectrochim Acta B 33B:241

    Google Scholar 

  26. MacDougall D, Crummett WB (1980) Anal Chem 52:2242

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors acknowledge the financial support from MEC (Project n°. BQU 2002-02872), partially co-financed by FEDER funds. J.L.F. also acknowledges a research fellowship from Spanish MEC.

Author information

Authors and Affiliations

  1. Department of Physical and Analytical Chemistry, Faculty of Experimental Sciences, University of Jaén, Paraje Las Lagunillas, s/n, 23071, Jaén, Spain

    Javier López-Flores, María L. Fernández-de Córdova & Antonio Molina-Díaz

Authors
  1. Javier López-Flores
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. María L. Fernández-de Córdova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Antonio Molina-Díaz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to María L. Fernández-de Córdova.

Rights and permissions

Reprints and permissions

About this article

Cite this article

López-Flores, J., Fernández-de Córdova, M.L. & Molina-Díaz, A. Determination of sub-ppb reserpine by an optosensing device based on photochemically induced fluorescence. Anal Bioanal Chem 388, 1771–1777 (2007). https://doi.org/10.1007/s00216-007-1384-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-007-1384-y

Keywords

Search

Navigation