Abstract
Protein can greatly enhance the fluorescence of curcumin (CU) in the presence of sodium dodecyl benzene sulfonate (SDBS). Experiments indicate that under the optimum conditions, the enhanced intensity of fluorescence is proportional to the concentration of proteins in the range of 0.0050–20.0 μg mL−1 for bovine serum albumin (BSA), 0.080–20.0 μg mL−1 for human serum albumin (HSA), and 0.040–28.0 μg mL−1 for egg albumin (EA). Their detection limits (S/N=3) are 1.4 ng mL−1, 20 ng mL−1, and 16 ng mL−1, respectively. The method has been satisfactorily used for the determination of proteins in actual samples. In comparison with most of fluorimetric methods, this method is quick and simple, has high sensitivity and good stability. The interaction mechanism is also studied.
Similar content being viewed by others
References
Lowry OH, Rosebrough NJ, Farr AL, Randail RJ (1951) J Biol Chem 193:265
Bradford MM (1976) Anal Biochem 72:248
Ohsawa K, Ebata N (1983) Anal Biochem 135:409
Saz JM, Krattiger B, Bruno AE, Díez-Masa JC (1995) J Chromatogr A 699:315
Wang SJ, Tseng WL, Lin YW, Chang HT (2002) J Chromatogr A 979:261
Chen YJ, Yang JH, Wu X, Wu T, Luan YX (2002) Talanta 58:869
Liu RT, Yang JH, Wu X, Lan ZJ (2002) Spectrochimi Acta A 58:3077
Ma CQ, Li KA, Tong SY (1997) Anal Chim Acta 338:255
Huang CB, Ci YX, Chang WB (2001) Chin J Anal Sci 17:186
Koller M, Eckert H (1997) Anal Chim Acta 352:31
Larive CK, Lunte SM, Zhong M, Perkins MD, Wilson GS, Gokalvangan G, Williams T, Afroz F, Schoneich C, Derick TS, Middaugh CR, Bodanowich-Knipp S (1999) Anal Chem 71:389
Sun CX, Yang JH, Li L, Wu X, Liu Y, Liu SF (2004) J Chromatogr B 803:173
Bggertson MJ, Craig DB, Biomed (2000) Chromatogr 14:156
Bank PR, Trends (1998) Anal Chem 17612
Krishnaswamy K, Raghuramulu N (1998) Indian J Med Res 108:167
Miquel J, Bernd A, Sempere JM, Diaz-Alperi J, Ramirez A (2002) Arch Gerontol Geriat 34:37
Ci YX, Chen L (1988) Analyst 113:679
Li KA, Tong SY (1996) Anal Biochem 233:151
Ma Q, Li KA, Tong SY (1996) Anal Chim Acta 333:83
Li DH, Yang HH, Zheng H, Chen B, Chen QY, Xu JG (1999) Chin J Anal Chem 27:1018
Qin WW, Gong GQ, Song YM (2000) Spectrochim Acta A 56:1021
Qin WW, Bao YJ, Gong GQ, Wu SF, Lu JL (2000) Chin J Anal Chem 28:526
Lee SH, Suh JK, Li M (2003) Bull Korean Chem Soc 24:45
Liu SF, Yang JH, Wu X, Wang F, Wang F, Jia Z, Mao LJ (2004) J Luminescence 19:352
Jiang CQ, Luo L (2004) Anal Chim Acta 506:171
Edwards HE, Thomas JK (1978) Carbohydr Res 65:173
Nakajima A (1983) Spectrochim Acta A 39:913
Chen GZ, Huang XZ, Zheng ZZ (1990) Fluorescence Anal Methods, Beijing, Science Press: 15
Clegg RM (1995) Curr Opin Biotech 6:103
Kumar GA, Unnikrishnan NV (2001) J Photochem Photobiol A 144:107
Dexter DL (1953) J Chem Phys 21:836
Eisenthal KB, Siegel S (1964) J Chem Phys 41:652
Acknowledgements
The Natural Science Foundations of China (20575035) and Shandong Province (Y2003B02), and a Visiting Scholar Foundation of the Key Laboratory at Shandong University supported this work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, F., Yang, J., Wu, X. et al. Fluorescence enhancement of the protein–curcumin–sodium dodecyl benzene sulfonate system and protein determination. Anal Bioanal Chem 385, 139–145 (2006). https://doi.org/10.1007/s00216-006-0372-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00216-006-0372-y