Localisation of mRNA for h5-HT_1B and h5-HT_1D receptors in human dorsal raphe

Abstract.

In the mammalian mesencephalon, virtually all serotoninergic neurons are located in the raphe nuclei and the adjacent reticular formation. Pharmacological evidence obtained in rodents suggests that terminal and somatodendritic autoreceptors controlling serotonin (5-hydroxytryptamine, 5-HT) release belong to the 5-HT_1B/D subtype of receptors, whereas somatodendritic autoreceptors controlling neuronal cell firing are predominantly of the 5-HT_1A subtype. This study investigated the presence of h5-HT_1D and h5-HT_1B receptor mRNA within the subdivisions of the dorsal raphe of post-mortem human brains by means of in situ hybridisation. Although differences in the labelling intensity, which may be caused by different pre- and/or post-mortem conditions, were obvious among the specimens, all brains expressed both the h5-HT_1D and the h5-HT_1B mRNA in dorsal raphe neurons. In comparison to h5-HT_1D mRNA, expression of h5-HT_1B mRNA was slightly more abundant. Information on the existence and localisation of h5-HT_1D and h5-HT_1B receptors in human dorsal raphe neurons confirms that both subtypes may serve an autoreceptor function in humans. This finding is of pharmacological relevance since these receptors are potential new targets for therapeutic interventions in psychiatric disorders such as depression and anxiety.