Abstract
Vibrio cholerae is the causative agent of water-borne diarrheal disease, cholera. The formation of biofilm favors survival and persistence of V. cholerae in the aquatic environment and also inside the host. AHL lactonase (AiiA), a metallo-beta-lactamase produced by Bacillus spp., blocks quorum sensing in Gram-negative bacteria by hydrolyzing N-acyl-homoserine lactones (AHLs). In the present investigation, AiiA-mediated inhibition of V. cholerae biofilm was studied. Two novel alleles of aiiA-encoding genes from Bacillus spp. were expressed in E. coli, and the results demonstrated that AiiA enzyme is a potent inhibitor of V. cholerae biofilm.
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Acknowledgments
This study was supported by an intramural support from Rajiv Gandhi Centre for Biotechnology, Trivandrum, India. Praveen Kumar gratefully acknowledges the Council of Scientific and Industrial Research, Govt. of India, for research fellowship. We are grateful to Dr. Saumya Raychaudhuri, Institute of Microbial Technology, Chandigarh, India, for providing V. cholerae strain PL91 used in this study. The authors are thankful to Prof. M. Radhakrishna Pillai, Director, RGCB for the facilities provided.
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Communicated by Jorge Membrillo-Hernandez.
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Augustine, N., Kumar, P. & Thomas, S. Inhibition of Vibrio cholerae biofilm by AiiA enzyme produced from Bacillus spp.. Arch Microbiol 192, 1019–1022 (2010). https://doi.org/10.1007/s00203-010-0633-1
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DOI: https://doi.org/10.1007/s00203-010-0633-1