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Comparison of the effects of alendronate sodium and calcitonin on bone–prosthesis osseointegration in osteoporotic rats

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Abstract

Summary

Alendronate (ALO) and calcitonin (CT), as commonly used antiosteoporosis drugs in current clinical practice, have been experimentally confirmed to produce the effectiveness of promoting osseointegration at the interface between prosthesis and host bone and enhancing the long-term stability of the prosthesis. Our current study compared these two drugs' effects on the osseointegration of prosthesis and found that both of them could promote bone attachment between prosthesis and host bone; moreover, ALO produced more pronounced effectiveness.

Introduction

A series of findings confirmed that ALO and CT improved bone attachment of implant in animals. However, which one shows stronger effectiveness has not yet been reported by previous researches. Our study compared the effects of the two commonly used antiosteoporosis drugs on the bone–prosthesis osseointegration so as to provide valuable reference for current clinical options of medication.

Methods

Forty female SD rats aged 5 months were randomly set into A, B, C, and D groups. Except for group A, the others were ovariectomized to establish osteoporosis model (lumbar bone mineral density (BMD) decreased by 20% 4 weeks after ovariectomy). All the rats received prosthesis implantation at their tibial plateau. Then, the rats in groups C and D were given ALO (7 mg/kg/w) orally and CT (5 IU/kg/day) subcutaneously for 12 weeks, respectively. Prior to the execution, application of tetracycline hydrochloride for staining in vivo was done. After harvesting and embedding, the tibia with implants were cut into thin slides, then the bone histomorphometry was measured to observe the new bone around prosthesis and to calculate the osseointegration rate of the implants. By comparison, the effect of the two drugs on osseointegration was evaluated.

Results

(1) Both ALO and CT can effectively enhance the volume of bone mass surrounding the hydroxyapatite (HA) prosthesis and also significantly lever up osseointegration rate to 63.7% and 45.7%, respectively (p < 0.05). However, ALO produced more periprosthesis osseointegration rate than CT, with difference of 18% (p < 0.05). (2) The rats' lumber BMD increased in both ALO and CT groups, from 0.081 ± 0.009 and 0.078 ± 0.009 to 0.116 ± 0.008 and 0.109 ± 0.010 g/cm2, respectively. Moreover, the effect of ALO was observed more pronounced than that of CT.

Conclusions

In osteoporotic conditions, both administration of ALO orally and CT subcutaneously can enhance periprosthesis bone mass and the effects on osseointegration between host bone and prosthesis. Compared with CT, the effect of ALO is more pronounced.

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References

  1. Karsdal MA, Henriksen K, Arnold M et al (2008) Calcitonin: a drug of the past or for the future? Physiologic inhibition of bone resorption while sustaining osteoclast numbers improves bone quality. BioDrugs 22(3):137–144

    Article  CAS  PubMed  Google Scholar 

  2. Viera-Negrón YE, Ruan WH, Winger JN et al (2008) Effect of ovariectomy and alendronate on implant osseointegration in rat maxillary bone. J Oral Implantol 34(2):76–82

    Article  PubMed  Google Scholar 

  3. Giro G, Gonçalves D, Sakakura CE et al (2008) Influence of estrogen deficiency and its treatment with alendronate and estrogen on bone density around osseointegrated implants: radiographic study in female rats. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105(2):162–167

    Article  PubMed  Google Scholar 

  4. Jensen TB, Bechtold JE, Chen X et al (2007) Systemic alendronate treatment improves fixation of press-fit implants: a canine study using nonloaded implants. J Orthop Res 25(6):772–778

    Article  CAS  PubMed  Google Scholar 

  5. Frenkel SR, Jaffe WL, Valle CD et al (2001) The effect of alendronate (Fosamax) and implant surface on bone integration and remodeling in a canine model. J Biomed Mater Res 58(6):645–650

    Article  CAS  PubMed  Google Scholar 

  6. Nociti FH Jr, Sallum AW, Sallum EA, Duarte PM (2002) Effect of estrogen replacement and calcitonin therapies on bone around titanium implants placed in ovariectomized rats: a histometric study. Int J Oral Maxillofac Implants 17(6):786–792

    PubMed  Google Scholar 

  7. Duarte PM, César-Neto JB, Sallum AW, Sallum EA, Nociti FH Jr (2003) Effect of estrogen and calcitonin therapies on bone density in a lateral area adjacent to implants placed in the tibiae of ovariectomized rats. J Periodontol 74(11):1618–1624

    Article  CAS  PubMed  Google Scholar 

  8. Januário AL, Sallum EA, de Toledo S, Sallum AW, Nociti JF Jr (2001) Effect of calcitonin on bone formation around titanium implant. A histometric study in rabbits. Braz Dent J 12(3):158–162

    PubMed  Google Scholar 

  9. Chen B-L, Liao W-M, Li F-B, Fu M, Xu D-L (2001) Effect of light weight-bearing activity on bone mineral density, histomorphometry and biomechanics in ovarietomized rats (chinese). Acd J SUMS 22(3):187–191

    CAS  Google Scholar 

  10. Chacon GE, Stine EA, Larsen PE, Beck FM, McGlumphy EA (2006) Effect of alendronate on endosseous implant integration: an in vivo study in rabbits. J Oral Maxillofac Surg 64(7):1005–1009

    Article  PubMed  Google Scholar 

  11. Duarte PM, de Vasconcelos Gurgel BC, Sallum AW, Filho GR, Sallum EA, Nociti FH Jr (2005) Alendronate therapy may be effective in the prevention of bone loss around titanium implants inserted in estrogen-deficient rats. J Periodontol 76(1):107–114

    Article  CAS  PubMed  Google Scholar 

  12. Nociti FH Jr, Sallum EA, Toledo S, Newman HN, Sallum AW (1999) Effect of calcitonin on bone healing following titanium implant insertion. J Oral Sci 41(2):77–80

    CAS  PubMed  Google Scholar 

  13. Giro G, Sakakura CE, Gonçalves D, Pereira RM, Marcantonio E Jr, Orrico SR (2007) Effect of 17beta-estradiol and alendronate on the removal torque of osseointegrated titanium implants in ovariectomized rats. J Periodontol 78(7):1316–1321

    Article  PubMed  Google Scholar 

  14. Kalu DN (1991) The ovariectomized rat model of postmenopausal bone loss. Bone Miner 15(3):175–191

    Article  CAS  PubMed  Google Scholar 

  15. Licata AA (1997) Bisphosphonate therapy. Am J Med Sci 313:17–22

    Article  CAS  PubMed  Google Scholar 

  16. Im GI, Qureshi SA, Kenney J et al (2004) Osteoblast proliferation and maturation by bisphosphonates. Biomaterials 25(18):4105–4115

    Article  CAS  PubMed  Google Scholar 

  17. Romanello M, Bivi N, Pines A et al (2006) Bisphosphonates activate nucleotide recep tors signaling and induce the expression of Hsp90 in osteoblast-like cell lines. Bone 39(4):739–753

    Article  CAS  PubMed  Google Scholar 

  18. Mehlhorn AT, Rechl H, Gradinger R et al (2008) Alendronate decreases TRACP 5b activity in osteoarthritic bone. Eur J Med Res 13(1):21–25

    CAS  PubMed  Google Scholar 

  19. Nenonen A, Cheng S, Ivaska KK et al (2005) Serum TRACP 5b is a useful marker for monitoring alendronate treatment: comparison with other markers of bone turnover. J Bone Miner Res 20(10):1804–1812

    Article  CAS  Google Scholar 

  20. Apone S, Lee MY, Eyre DR (1997) Osteoclasts generate cross-linked collagen N-telopeptides (NTx) but not free pyridinolines when cultured on human bone. Bone 21(2):129–136

    Article  CAS  PubMed  Google Scholar 

  21. Byrjalsen I, Leeming DJ, Qvist P et al (2007) Bone turnover and bone collagen maturation in osteoporosis: effects of antiresorptive therapies. Osteoporos Int 19(3):339–348

    Article  PubMed  Google Scholar 

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Acknowledgements

This work is supported by Guangdong Provincial Science and Technology Program 2007B312004 (to B.-L. Chen).

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Authors and Affiliations

  1. Department of Orthopaedics, First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Second Road, Guangzhou, 510080, China

    B.-L. Chen, D.-H. Xie, Z.-M. Zheng, Y.-Q. Li, F.-B. Li & W.-M. Liao

  2. Department of Orthopaedics and Traumatology, University of Hong Kong, Hong Kong, China

    W. Lu

  3. Department of Biomaterial, South China University of Science and Technology, 1 Wushan Road, Guangzhou, 510000, China

    C.-Y. Ning

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  1. B.-L. Chen
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  2. D.-H. Xie
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  3. Z.-M. Zheng
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  4. W. Lu
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  5. C.-Y. Ning
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  6. Y.-Q. Li
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  7. F.-B. Li
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  8. W.-M. Liao
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Correspondence to B.-L. Chen.

Additional information

Dr B.-L. Chen and D.-H. Xie contributed equally to this work.

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Chen, BL., Xie, DH., Zheng, ZM. et al. Comparison of the effects of alendronate sodium and calcitonin on bone–prosthesis osseointegration in osteoporotic rats. Osteoporos Int 22, 265–270 (2011). https://doi.org/10.1007/s00198-010-1186-5

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  • DOI: https://doi.org/10.1007/s00198-010-1186-5

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