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Strain-specific and recessive QTLs involved in the control of partial resistance to Fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon

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Abstract

Fusarium oxysporum f. sp. melonis (FOM) causes serious economic losses in melon (Cucumis melo L.). Two dominant resistance genes have been identified, Fom-1 and Fom-2, which provide resistance to races 0 and 2 and races 0 and 1, respectively, however FOM race 1.2 overcomes these resistance genes. A partial resistance to FOM race 1.2 that has been found in some Far East accessions is under polygenic control. A genetic map of melon was constructed to tag FOM race 1.2 resistance with DNA markers on a recombinant inbred line population derived from a cross between resistant (Isabelle) and susceptible (cv. Védrantais) lines. Artificial root inoculations on plantlets of this population using two strains, one that causes wilting (FOM 1.2w) and one that causes yellowing (FOM 1.2y), resulted in phenotypic and genotypic data that enabled the identification of nine quantitative trait loci (QTLs). These QTLs were detected on five linkage groups by composite interval mapping and explained between 41.9% and 66.4% of the total variation. Four digenic epistatic interactions involving seven loci were detected and increased the total phenotypic variation that was explained. Co-localizations between QTLs and resistance gene homologs or resistance genes, such as Fom-2 and Vat, were observed. A strain-specific QTL was detected, and some QTLs appeared to be recessive.

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Acknowledgements

The authors thank Benoît Moury for his critical review of the manuscript. Laure Perchepied thanks the ASL, Clause-Tézier, Gautier, Rijk Zwaan, Seminis and Takii seed companies for their technical assistance and, together with ANRT, for their financial support. The authors thank Nathalie Giovinazzo, Virginie Chareyron and Didier Besombes for their technical assistance.

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  1. Institut National de la Recherche Agronomique, Unité de Génétique et d’Amélioration des Fruits et Légumes, BP 94, 84143, Montfavet Cedex, France

    L. Perchepied, C. Dogimont & M. Pitrat

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  1. L. Perchepied
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  3. M. Pitrat
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Correspondence to M. Pitrat.

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Communicated by I. Paran

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Perchepied, L., Dogimont, C. & Pitrat, M. Strain-specific and recessive QTLs involved in the control of partial resistance to Fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon. Theor Appl Genet 111, 65–74 (2005). https://doi.org/10.1007/s00122-005-1991-y

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