Abstract
Where differences have been reported between tumor and normal mitochondrial DNA (mtDNA), they have generally involved limited modifications of the genome (Tairaet al., Nucleic Acids Res. 11:1635, 1983; Shay and Werbin,Mutat. Res. 186:149, 1987). However, Corralet al. (Nucleic Acids Res. 16:10935, 1988;17:5191, 1989) observed recombination between cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 6 (ND6), two genes normally on opposite sides of the circular mitochondrial genome. In rat hepatoma mtDNA COI and ND6 were reported to be separated by only 230 base pairs (Corralet al., 1988, 1989). We have performed RFLP analysis on mtDNA from normal rat livers and rat hepatomas, using COI and ND6 probes. Additional experiments compared end-labeled DNA fragments produced byEcoRI andHindIII digestion of mtDNA. These studies failed to provide any evidence for genetic recombination in rat hepatoma mtDNA, even in the same cell line used by Corralet al. Rather, they support the conclusion that mtDNA from tumor and normal tissues exhibits a low degree of heterogeneity.
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This work was supported in part by Grant 003644-010 from the State of Texas Advanced Research Program and by fellowships awarded to W.C.W. from the Biotechnology Institute of Texas Tech University.
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Nakashima, R.A., Li, X., Bayouth, J.M. et al. Restriction fragment length polymorphism (RFLP) analysis provides evidence for a high degree of homology of mitochondrial DNAs from rat hepatomasVersus normal rat livers. Biochem Genet 30, 545–556 (1992). https://doi.org/10.1007/PL00020516
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DOI: https://doi.org/10.1007/PL00020516