Summary
The electron microscopy cytochemical detection of phospholipids in well-defined areas in the interphase nuclei of hepatocytes has been obtained by the acid haematein test, modified for electron microscopy and by the phospholipase A2-colloidal gold method. The specificity of both methods were controlled by enzymatic digestion with phospholipase. The main intra-nuclear localization of phospholipids is at the border between the condensed and dispersed chromatin, where non-ribosomal RNA is also revealed by RNase-gold labelling. Phospholipids are detected, too, over the clusters of interchromatin granules and in the fibrillar component of the nucleolus.
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Fraschini, A., Albi, E., Gahan, P.B. et al. TEM cytochemical study of the localization of phospholipids in interphase chromatin in rat hepatocytes. Histochemistry 97, 225–235 (1992). https://doi.org/10.1007/BF00267632
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DOI: https://doi.org/10.1007/BF00267632