Skip to main content
SpringerLink
Log in

Effect of pulse frequency and amplitude of D-Trp6-luteinizing hormone-releasing hormone on the pulsatile secretion of prolactin and LH

  • Published:
Journal of Endocrinological Investigation Aims and scope Submit manuscript

Abstract

This work analyzes the effect of the pulse amplitude and frequency of a potent LHRH analog, D-Trp6-LHRH, in a perfusion system of isolated rat pituitary cells. To this purpose, we studied the LH and PRL secretion in different conditions: basal secretion, secretion after increasing concentrations of D-Trp6-LHRL (0.001, 0.01, 0.1 and 1 nM) secretion in function of the pulses frequency (2,3, and 4 pulses per h) and amplitude (0.1, 1 and 10nM). The principal findings were: 1. The basal LH and PRL secretions was pulsatile; 2. The stimulation of LH by the analog was not dose-dependent; 3. When more than 2 pulses per h were administered, a rapid desensitization of gonadotroph to release LH (at 20–30 min) occurred; 4. There was a loss of pulsatility of PRL secretion with an increase in the pulse frequency and amplitude of the D-Trp6-LHRH, which was produced parallelly to the desensitization of the gonadotroph to release LH. In summary, these findings suggest that a rapid loss of pulsatility of the PRL when the D-Trp6-LHRH pulse frequency and amplitude is increased might be due to the early desensitization of the gonadotroph to the analog.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Horvarts E., Kovacs K., Ezrin C. Functional contact between lactotrophs and gonadotrophs in rat pituitary. IRCS. Med. Sci. 5; 511, 1970.

    Google Scholar 

  2. Nakane P.K. Classification of pituitary cell types with immunoenzyme histochemistry. J. Histochem. Cytochem. 18: 9, 1970.

    Article  PubMed  CAS  Google Scholar 

  3. Casper R.F., Yen S.S.C. Simultaneous pulsatile release of prolactin and luteinizing hormone induced by luteinizing hormone releasing factor agonist. J. Clin. Endocrinol. Metab. 52: 934, 1981.

    Article  PubMed  CAS  Google Scholar 

  4. Mais V., Yen S.S.C. Prolactin-releasing action of gonadotropin-releasing hormone in hypogonadal women. J. Clin. Endocrinol. Metab. 62: 1089, 1986.

    Article  PubMed  CAS  Google Scholar 

  5. Wildt L., Hansler A., Marshall G., Knobil E. GnRH has prolactin releasing activity. Fed. Proc. 39:374, 1980.

    Google Scholar 

  6. Denef C., Andries M. Evidence for a paracrine interaction between gonadotrophs and lactotrophs in pituitary cell aggregates. Endocrinology 116:813, 1983.

    Article  Google Scholar 

  7. Debeljuk L., Torres-Aleman I., Schally A.V. D-Trp-6-Luteinizing hormone releasing hormone inhibits hyperprolactinemia in female rats. Endocrinology 116:2227, 1985.

    Article  PubMed  CAS  Google Scholar 

  8. Rubio M.A., Torres-Aleman I., Calle J.R., Cabranes J.A., Schally A.V., Charro A.L. D-Trp-6-Luteinizing hormone-releasing hormone inhibits Sulpiride-induced hyperprolactinemia in normal men. J. Clin. Endocrinol. Metab. 65: 368, 1987.

    Article  PubMed  CAS  Google Scholar 

  9. Torres-Aleman I., Redding T.W., Schally A.V. Inhibition of growth of a prolactin and growth hormone secreting pituitary tumor in rats by D-Tryptophan-6-analog of luteinizing hormone releasing hormone. Proc. Natl. Acad. Sci. USA. 82:1252, 1985.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  10. Rubio M.A., Cabranes J.A., Schally A.V., Charro A.L. Prolactin lowering effect of luteinizing hormone-releasing hormone agonist administration in prolactinoma patients. J. Clin. Endocrinol. Metab. 69:444, 1989.

    Article  PubMed  CAS  Google Scholar 

  11. Evans W.S., Cronin M.J., Thorner M.O. Continuous perifusion of dispersed anterior pituitary cells. Technical aspects. Meth. Enzymol. 103:294, 1983.

    Article  PubMed  CAS  Google Scholar 

  12. Santen R.J., Bardin C.W. Episodic luteinizing hormone secretion in man. Pulse analysis, clinical interpretation, physiological mechanism. J.Clin. Invest. 52: 2617, 1973.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  13. Fox S.R., Smith M.S. Changes in the pulsatile pattern of luteinizing hormone secretion during the oestrous cycle. Endocrinology 116:1485, 1985.

    Article  PubMed  CAS  Google Scholar 

  14. Pohl C.R., Weiner R.I., Smith M.S. Relation between luteinizing hormone and prolactin pulses in ovariectomized rats with or without dopamine inhibition. Endocrinology 123:1591, 1988.

    Article  PubMed  CAS  Google Scholar 

  15. Ben-Jonathan N. Dopamine: A prolactin-inhibiting hormone. Endocr. Rev. 6:564, 1985.

    Article  PubMed  CAS  Google Scholar 

  16. Torres-Aleman I., Fernandez M., Debeljuk L., Charro A.L. Effect of D-Trp-6-LHRH infusion on prolactin secretion by perifused rat pituitary cells. Regul. Peptides 18:19, 1987.

    Article  CAS  Google Scholar 

  17. Kamel F., Balz J.A., Kubajak C.L., Schneiner V.A. Effects of luteinizing hormone (LH)-releasing hormone pulse amplitude and frequency on LH secretion by perifused rat anterior pituitary cells. Endocrinology 120:1644, 1987.

    Article  PubMed  CAS  Google Scholar 

  18. Kamel F., Balz J.A., Kubajak C.L., Schneiner V.A. Gonadal steroids modulate pulsatile luteinizing hormone secretion by perifused rat anterior pituitary cells. Endocrinology 120: 1651, 1987.

    Article  PubMed  CAS  Google Scholar 

  19. Conn P.M., Crowley W.F. Gonadotropin-releasing hormone and its analogues. N. Engl. J. Med. 324: 93, 1991.

    Article  PubMed  CAS  Google Scholar 

  20. Naor Z., Clayton R.N., Catt K.J. Characterization of gonadotropin-releasing hormone receptors in cultured rat pituitary cells. Endocrinology 107:1144, 1980.

    Article  PubMed  CAS  Google Scholar 

  21. King J.A., Davidson J.S., Millar R.P. Desensitization to gonadotropin-releasing hormone in perifused chicken anterior pituitary cells. Endocrinology 119:1510, 1986.

    Article  PubMed  CAS  Google Scholar 

  22. Buckingham J.C., Cover P.O. Changes in the responsiveness of perifused rat adenohypophysial cells to repeat stimulation with luteinizing hormone releasing hormone. Acta Endocrinol. (Copenh.) 113:479, 1986.

    CAS  Google Scholar 

  23. Tsuei-Chu-Lin, Jackson G.L. Long term superfusion of rat anterior pituitary cells: Effects of repeated pulses of gonadotropin-releasing hormone at different doses, durations and frequencies. Endocrinology 115:605, 1984.

    Article  Google Scholar 

  24. Gorospe W.E., Conn P.M. Agents that decreased gonadotropin-releasing hormone (GnRH) receptor internalization do not inhibit GnRH-mediated gonadotroph desensitization. Endocrinology 120:222, 1987.

    Article  PubMed  CAS  Google Scholar 

  25. Denef C. Paracrine interactions in the anterior pituitary. Clin. Endocrinol. Metab. 15:1, 1986.

    Article  PubMed  CAS  Google Scholar 

  26. Kubota T., Judd A.M., Mc Leod R.M. The paracrine role of angiotensin in gonadotropin-releasing hormone stimulated prolactin release in rats. J. Endocrinol. 125:225, 1990.

    Article  PubMed  CAS  Google Scholar 

  27. Denef C., Tilemans D., Andries M. Paracrine control in the anterior pituitary: Growth factors from gonadotrophs control DNA replication lactotrophs, somatotrophs and corticotrophs. In: Faglia G., Beck-Pecoz P., Ambrosi B., Travaglini P., Spada A. (Eds.), Pituitary Adenomas: New trends in basic and clinical research. Elsevier Science Publishers B.V., Amsterdam, 1991, Vol. 1, p. 49.

    Google Scholar 

  28. Blackwell R.E., Garrison P.N. Inhibition of prolactin secretion by antiserum to the α and \ subunit genes. Am. J. Obstet. Gynecol. 156: 863, 1987.

    Article  PubMed  CAS  Google Scholar 

  29. Wierman M.E., Gharib S.D., Chin W.W. The structure and regulation of the pituitary gonadotropin subunit genes. Clin. Endocrinol. Metab. 4: 869, 1988.

    Google Scholar 

  30. Lambers S.W.J., Uitterlinen P., Zuiderwijk-Roest J.M., Bons-Van-Evelingen E.G., De Jong F. Effects of a luteinizing hormone-releasing hormone analog and tamoxifen on the growth of an estrogen induced prolactin-secreting rat pituitary tumor and its influence on pituitary gonadotropins. Endocrinology 108:1878, 1981.

    Article  Google Scholar 

  31. Herbert D.C., Rennels E.G. Effect of synthetic luteinizing hormone releasing hormone on prolactin secretion from clonal pituitary cells. Biochem. Biophys. Res. Commun. 79:133, 1977.

    Article  PubMed  CAS  Google Scholar 

  32. Begeot M., Hemmins F.J., Martinat N., Dubois M.P., Dubois P.M. Gonadotropin releasing hormone (GnRH) stimulates immunoreactive lactotroph differentiation. Endocrinology 112:2224, 1983.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Endocrinology Service, University Hospital of San Carlos, C/ Martin Lagos s/n, 28040, Madrid, Spain

    T. Rodriguez, E. Bordiu, J. A. Rubio, A. Duran & A. L. Charro

Authors
  1. T. Rodriguez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Bordiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. A. Rubio
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Duran
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. L. Charro
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodriguez, T., Bordiu, E., Rubio, J.A. et al. Effect of pulse frequency and amplitude of D-Trp6-luteinizing hormone-releasing hormone on the pulsatile secretion of prolactin and LH. J Endocrinol Invest 16, 601–607 (1993). https://doi.org/10.1007/BF03347679

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03347679

Key-words

Search

Navigation