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Fasting and post-prandial adipose tissue lipoprotein lipase and hormone-sensitive lipase in obesity and Type 2 diabetes

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Abstract

Background: Fasting and post-prandial abnormalities of adipose tissue (AT) lipoprotein lipase (LPL) and hormone-sensitive lipase (HSL) activities may have pathophysiological relevance in insulin-resistant conditions. Aim: The aim of this study was to evaluate activity and gene expression of AT LPL and HSL at fasting and 6 h after meal in two insulin-resistant groups — obese with Type 2 diabetes and obese without diabetes — and in non-diabetic normal-weight controls. Material/subjects and methods: Nine obese subjects with diabetes, 10 with obesity alone, and 9 controls underwent measurements of plasma levels of glucose, insulin, and triglycerides before and after a standard fat-rich meal. Fasting and post-prandial (6 h) LPL and HSL activities and gene expressions were determined in abdominal subcutaneous AT needle biopsies. Results: The diabetic obese subjects had significantly lower fasting and post-prandial AT heparin-releasable LPL activity than only obese and control subjects (p<0.05) as well as lower mRNA LPL levels. HSL activity was significantly reduced in the 2 groups of obese subjects compared to controls in both fasting condition and 6 h after the meal (p<0.05), while HSL mRNA levels were not different. There were no significant changes between fasting and 6 h after meal measurements in either LPL or HSL activities and gene expressions. Conclusions: Lipolytic activities in AT are differently altered in obesity and Type 2 diabetes being HSL alteration associated with both insulin-resistant conditions and LPL with diabetes per se. These abnormalities are similarly observed in the fasting condition and after a fat-rich meal.

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Authors and Affiliations

  1. Department of Clinical and Experimental Medicine, Federico II University, Via Pansini 5, 80131, Napoli, Italy

    G. Costabile, G. Annuzzi, L. Di Marino, C. De Natale, L. Bozzetto, P. Cipriano & A. A. Rivellese MD

  2. Institute of Food Science and Technology, CNR, Avellino

    R. Giacco

  3. National Centre for Food Quality and Risk Assessment, National Institute of Health, Rome, Italy

    C. Santangelo & R. Masella

Authors
  1. G. Costabile
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  2. G. Annuzzi
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  3. L. Di Marino
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  4. C. De Natale
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  5. R. Giacco
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  6. L. Bozzetto
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  7. P. Cipriano
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  8. C. Santangelo
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  9. R. Masella
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  10. A. A. Rivellese MD
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Correspondence to A. A. Rivellese MD.

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Costabile, G., Annuzzi, G., Di Marino, L. et al. Fasting and post-prandial adipose tissue lipoprotein lipase and hormone-sensitive lipase in obesity and Type 2 diabetes. J Endocrinol Invest 34, e110–e114 (2011). https://doi.org/10.1007/BF03347469

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