Abstract
Embryogenic calli were obtained within 3–4 weeks on MS medium supplemented with 3% sucrose, 200 mg I-1 caseinhydrolysate, and 3 mg l−1 each of 2,4-D and NAA from 3–4 days imbibed seeds. Heart-shaped embryos differentiated when growth regulators were withdrawn gradually. Quantification of somatic embryo formation showed a direct relationship between initial response, somatic embryo formation and its subsequent germination. The somatic embryos germinated into emblings with 90% conversion frequency on MS medium containing 2% sucrose.
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References
Srivastava SD, J Nat Prod, 49 (1986) 56.
Murthy BNS & Saxena PK, Plant Cell Rep, 17 (1998) 469.
Thakur R, Rao PS & Bapat VA, Plant Cell Rep, 18 (1998) 127.
Sujatha M & Chandran K, J Exp Biol, 35 (1997) 787.
Murashige T & Skoog F, Physiol Plant, 15 (1962) 473.
Chan JL, Saenz L, Talavera C, Hornung R, Robert M & Oropeza , Plant Cell Rep, 17 (1998) 515.
Tiwari V, Deo Singh B & Nath Tiwari K, Plant Cell Rep, 17 (1998) 538.
Ishii Y, Takamura T, Goi M & Tanaka M, Plant Cell Rep, 17 (1998) 446.
Chengalrayan K, Mhaske VB & Hazra S, Plant Cell Rep, 17 (1998) 522.
Gautam VK, Nanda K & Gupta SC, Plant Cell Tiss Org Cult, 34 (1993) 13.
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Deb, C.R. Somatic Embryogenesis and Plantlet Regeneration of Melia azedarach L (Ghora Neem) from Cotyledonary Segments. J. Plant Biochem. Biotechnol. 10, 63–65 (2001). https://doi.org/10.1007/BF03263110
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DOI: https://doi.org/10.1007/BF03263110