Abstract
cDNA was synthesized from RNA isolated from developing seeds of Brassica juncea cv Pusa Jai Kisan at 25–45 days after flowering and partial cDNA sequences encoding ω-3 desaturase gene were PCR amplified using specifically designed primers. The amplified products were cloned, and three of the clones subjected to partial sequence analysis. The clones showed high homology to ω-3 desaturase sequence from other sources. A genomic clone corresponding to ω-3 desaturase was isolated from the genomic library of B. juncea constructed in λEMBL-3 vector using one of the PCR clones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Knutzon DS, Thompson GA, Radke SE, Johnson WB, Knauf VC & Kridl C, Proc Natl Acad Sci, USA, 89, (1992), 2624.
Kinney AJ, Nature Biotechnol, 14, (1996), 946.
Voelker TA, Worrell AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Radke SE & Davies HM, Science, 257, (1992), 72.
Cahoon EB, Shanklin J & Ohlrogge JB, Proc Natl Acad Sci, USA, 89, (1992), 11184
Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HM & Somerville CR, Science, 258, (1992), 1353.
Baicells L, Swineburne J & Coupland G, Trends Biotechnol, 9, (1991), 31
Yadav NS, Wierzbieki A, Aegerter M, Caster CS, Perez-Grau L, Kinney AJ, Hitz WO, Booth JR Jr, Sehweiger B, Stecca KL, Allen SM, Blackwell M, Reiter RS, Carlson T J, Russell SH, Feldmann KA, Pierce J & Browse J, Plant Physiol, 103 (1993) 467.
Kirsch C, Takamiya-Wik M, Reinold S, Hahlbrock K & Somssich IE, Proc Natl Acad Sci. USA, 94, (1997), 2079.
Kartha ARS & Sethi AS, Ind J Agric Sci. 27 (1957), 211.
Morrison WR & Smith LM, J Lipid Res, 5, (1964), 600.
Sambrook J, Fritsch EF, & Maniatis T, Molecular cloning: A laboratory mannual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).
Sanger F, Nicklen, S & Coulson AR, Proc Nati Acad Sci, USA, 74, (1977), 5463.
Dellaporta SL, Wood J & Hicks JB, Plant Mol Biol Rep, 1, (1983), 19.
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA & Struhl K, Short protocols in molecular biology. 4th ed. John Wiley & Sons. Inc. (1999).
Altschul F, Gish W, Miller W, Myers EW & Lipman DJ, J Mol Biol. 215 (1990) 403.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Garg, R., Kiran, M.D., Santha, I.M. et al. Cloning of a Gene Sequence for co-3 Desaturase from Brassica juncea . J. Plant Biochem. Biotechnol. 9, 13–16 (2000). https://doi.org/10.1007/BF03263076
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263076