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Real-Time PCR Analysis of Trinucleotide Repeat Allele Expansions in the Androgen Receptor Gene

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Abstract

Introduction:The expansion of specific trinucleotide repeats results in certain genetic disorders.

Method: Real-time PCR analysis was used to rapidly discriminate between normal and expanded (CAG)n alleles in the androgen receptor gene.

Result: The difference in melting temperature (Tm) between the most common normal and expanded alleles was approximately 1°C.

Conclusion: Real-time PCR analysis seems to be a highly reliable and rapid method, which may facilitate the first molecular approach to human trinucleotide repeat disorders.

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Acknowledgements

No sources of funding were used to assist in conducting this study. The authors have no conflicts of interest that are directly relevant to the content of the study.

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Authors and Affiliations

  1. Genetics Unit, Department of Obstetrics and Gynaecology, Ioannina University School of Medicine, Ioannina, 45110, Greece

    Anthoula Chatzikyriakidou &  Ioannis Georgiou

  2. Department of Neurology, Eginition Hospital, Athens University School of Medicine, Athens, Greece

    Christos Yapijakis & Dimitrios Vassilopoulos

  3. Department of Urology, Ioannina University School of Medicine, Ioannina, Greece

    Nikolaos Sofikitis

Authors
  1. Anthoula Chatzikyriakidou
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  2. Christos Yapijakis
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  3. Nikolaos Sofikitis
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  4. Dimitrios Vassilopoulos
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  5. Ioannis Georgiou
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Correspondence to Ioannis Georgiou.

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Chatzikyriakidou, A., Yapijakis, C., Sofikitis, N. et al. Real-Time PCR Analysis of Trinucleotide Repeat Allele Expansions in the Androgen Receptor Gene. CNS Drugs 9, 217–219 (2005). https://doi.org/10.1007/BF03260095

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  • DOI: https://doi.org/10.1007/BF03260095

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