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Formation of glucoside conjugate of acetaminophen by fungi separated from soil

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Summary

The phase II metabolite of acetaminophen in filamentous fungi and actinomycetes separated from soil was investigated. Fifty-four filamentous fungi and twenty-seven actinomycetes were screened to transform acetaminophen. The metabolites of acetaminophen were assayed using liquid chromatography-tandem mass spectrometry. The only metabolite was subject to enzymatic hydrolysis to confirm its structure. Acetaminophen was converted into glucoside conjugate, by filamentous fungi JX1-60, LN17-2, LN20-1 and the yield of the conjugate was 60.01%, 44.27%, 100%, respectively, and no phase I metabolites were detected. Glucoside conjugation of acetaminophen in filamentous fungi differs from the phase II metabolism of glucuronidation in humans. The fungus LN20-1 could be a suitable model to synthesize glucoside conjugate of acetaminophen.

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Authors and Affiliations

  1. Shanghai Drug-Metab Biotech Co., Ltd, 801 Yinghua Road, Mailbox 23-1202, 201204, Shanghai, People’s Republic of China

    H. H. Huang

  2. Shenyang Pharmaceutical University, Shenyang, P.R. China

    L. H. Lin, P. Zhang & X. L. Qi

  3. Center for Drug Metabolism Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, P.R. China

    D. F. Zhong

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  1. H. H. Huang
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  2. L. H. Lin
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  3. P. Zhang
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  4. X. L. Qi
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  5. D. F. Zhong
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Huang, H.H., Lin, L.H., Zhang, P. et al. Formation of glucoside conjugate of acetaminophen by fungi separated from soil. European Journal of Drug Metabolism and Pharmacokinetics 31, 103–108 (2006). https://doi.org/10.1007/BF03191126

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  • DOI: https://doi.org/10.1007/BF03191126

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