Abstract
Classical swine fever virus (CSFV) is the pathogen of the swine fever. Understanding of the replication and expression of its genome is the basis for research of the pathogenicity for CSFV and development of antiviral drug. The noncoding regions (NCRs) of CSFV are the main regulatory regions for replication and expression. Qualitative, quantitative and structural analysis of 3′ NCRs and 5′ NCRs was done in order to locate the regulatory region in the NCRs and to character the NCRs. The sites, conserved sequences and structural elements related to the initiation of replication and expression were extracted from 17 3′ NCRs and 56 5′ NCRs. Those ciselements may be initial recognition sites for replication, binding sites for transcription factors of host cell and interacting sites for initiation of protein synthesis, based on which a mechanism for the replication and expression of CSFV was brought forth. This research offers the direction for further experiment and lays down a basis for the research on hepatitis C virus (HCV), other pestiviruses and plus-strand RNA viruses.
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Xiao, M., Zhu, Z. & Zhang, C. Qualitative, quantitative and structural analysis of non-coding regions of classical swine fever virus genome. Chin. Sci. Bull. 46, 1251–1257 (2001). https://doi.org/10.1007/BF03184319
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DOI: https://doi.org/10.1007/BF03184319