Skip to main content
SpringerLink
Log in

Photosynthetic bicarbonate utilization inPorphyra haitanensis (Bangiales, Rhodophyta)

  • Notes
  • Published:
Chinese Science Bulletin

Abstract

The activities of carbonic anhydrase (CA) and photosynthesis ofPorphyra haitanensis were investigated in order to see its photosynthetic utilization of inorganic carbon source. Both intra- and extra-cellular CA activities existed in the thallus. CA inhibitors, acetazolamide (AZ) and ethoxy-zolamide (EZ), remarkably depressed the photosynthetic oxygen evolution in seawater of pH 8.2 and 10.0, and EZ showed stronger inhibition than AZ. The observed net photosynthetic rate in seawater of pH 8.2 was much higher than that of CO2 supply theoretically derived from spontaneous dehydration of HCO3 −.P. haitanensis also showed a rather high pH compensation point (9.9). The results demonstrated thatP. haitanensis could utilize bicarbonate as the external inorganic carbon source for photosynthesis. The bicarbonate utilization was closely associated with HCO3 − dehydration catalyzed by extracellular CA activity. The inorganic carbon composition in seawater could well saturate the photosynthesis ofP. haitanensis. The low Km value and compensation points for inorganic carbon reflected the existence of CO2-concentrating mechanism in this alga.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Raven, J. A., Inorganic carbon acquisition by marine autotrophs, Adv. Bot. Res., 1997, 27: 85.

    Article  Google Scholar 

  2. Falkowski, P. G., Raven, J. A., Aquatic Photosynthesis, Blackwell Science, Malden, MA: Capital City Press, 1997.

    Google Scholar 

  3. Hanelt, D., Huppertz, K., Nultsch, W., Daily course of photosynthesis and photoinhibition in marine macroalgae investigated in the laboratory and field, Mar. Ecol. Prog. Ser., 1993, 97: 31.

    Article  Google Scholar 

  4. Gao, K., McKinley, K. R., Use of macroalgae for marine biomass production and CO2 remediation: A review, J. Appl. Phycol., 1994, 6: 45.

    Article  Google Scholar 

  5. Larsson, C., Axelsson, L., Bicarbonate uptake and utilization in marine macroalgae, Eur. J. Phycol., 1999, 34: 79.

    Article  Google Scholar 

  6. Haglund, K., Bjork, M., Ramazanov, Z. et al., Role of external carbonic anhydrase in light-dependent alkalization byFucus serratus L. andLaminaria saccharina (L.) Lamour, (Phaeophyta), Planta, 1992, 188: 1.

    Article  Google Scholar 

  7. Yue, G. F., Wang, J. X., Wang, J. F. et al., Inorganic carbon acquisition by juvenile sporophyte of Laminarials (L. Japonica ×L. Longissima), Oceanol Limnol Sinica (in Chinese with English summary), 2001, 32(6): 647.

    Google Scholar 

  8. Zou, D., Gao, K., Progress in studies on photosynthetic inorganic carbon utilization in marine macroalgae, Marine Science Bulletin (in Chinese with English summary), 2001, 20(5): 83.

    Google Scholar 

  9. Axelsson, L., Ryberg, H., Beer, S., Two modes of bicarbonate utilization in the marine green macroalgaUlva lacturca, Plant Cell Environ., 1995, 18: 439.

    Article  Google Scholar 

  10. Larsson, C., Axelsson, L., Ryberg, H. et al., Photosynthetic carbon utilization byEnteromorpha intestinalis (Chorophyta) form a Swedish rockpool, Eur. J. Phycol., 1997, 32: 49.

    Article  Google Scholar 

  11. Axelsson, L., Larsson, C., Ryberg, H., Affinity, capacity and oxygen sensitivity of the two different mechanisms for bicarbonate utilization inUlva lactuca L. (Chlorophyta), Plant Cell Environ., 1999, 22: 969.

    Article  Google Scholar 

  12. Johnston, A. M., Maberly, S. C., Raven, J. A., The acquisition of inorganic carbon by four red macroalgae, Oecologia, 1992, 92: 317.

    Article  Google Scholar 

  13. Giordano, M., Maberly, S. C., Distribution of carbonic anhydrase in British marine macroalgae, Oecologia, 1989, 81: 534.

    Article  Google Scholar 

  14. Gao, K., Research techniques and methods in characterizing photosynthetic carbon fixation by algae, Marine Science (in Chinese), 1999(6): 37.

  15. Axelsson, L., Mercado, J., Figueroa, F., Utilization of HCO3 − at high pH by the brown macroalgaLaminaria saccharina, Eur. J. Phycol., 2000, 35: 53.

    Google Scholar 

  16. Moroney, J. V., Bartlett, S. G., Samuelsson, G., Carbonic anhydrases in plants and algae, Plant Cell Environ., 2001, 24: 141.

    Article  Google Scholar 

  17. Stumm, W., Morgan, J. J., Aquatic Chemistry, 3rd ed., New York: John Wiley and Sons, 1996.

    Google Scholar 

  18. Johnson, K. S., Carbon dioxide hydration and dehydration kinetics in seawater, Limol. Oceanogr., 1982, 27: 849.

    Article  Google Scholar 

  19. Axelsson, L., Uusitalo, J., Carbon acquisition strategies for marine macroalgae, Part I: Utilization of proton exchanges visualized during photosynthesis in a closed system, Mar. Biol., 1988, 97: 295.

    Article  Google Scholar 

  20. Maberly, S. C., Raven, J. A., Johnston, A. M., Discriminiation between12C and13C by marine plants, Oecologia, 1992, 91: 481.

    Article  Google Scholar 

  21. Mercado, J. M., Figuroa, F. L., Niell, F. X. et al., A new method for estimating external carbonic anhydrase activity in marcoalgae, J. Phycol., 1997, 33: 999.

    Article  Google Scholar 

  22. Kaplan, A., Reinhold, A., CO2 concentrating mechanism in photosynthetic microorganism, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1999, 50: 539.

    Article  Google Scholar 

  23. Maberly, S. C., Exogenous sources of inorganic carbon for photosynthesis by marine macroalgae, J. Phycol., 1990, 26: 439.

    Article  Google Scholar 

  24. Cook, C. M., Lanaras, T., Colman, B., Evidence for bicarbonate transport in species of red and brown macrophytic marine algae, J. Exp. Bot., 1986, 37: 977.

    Article  Google Scholar 

  25. Maberly, S. C., Carbonate ions appear to neither inhibit nor stimulate use of bicarbonate ions in photosynthesis byUlva lactuca, Plant Cell Environ., 1992, 15: 255.

    Article  Google Scholar 

  26. Kerby, N. W., Raven, J. A., Transport and fixation of inorganic carbon by marine algae, Adv. Bot. Res., 1985, 11: 71.

    Article  Google Scholar 

  27. Johnston, A. M., Raven, J. A., The utilization of bicarbonate ions by the macroalgaeAscophyllum nodosum (L.) Le Jolis, Plant Cell Environ., 1986, 9: 175.

    Google Scholar 

  28. Colman, B., The effect of temperature and oxygen on the CO2 compensation point of the marine algaUlva lactuca, Plant Cell Environ., 1984, 7: 619.

    Google Scholar 

  29. Smith, R. G., Bidwell, R. G. S., Mechanism of photosynthetic carbon dioxide uptake by the red macroalgaChondrus crispus, Plant Physiol., 1989, 89: 93.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Marine Biology Institute, Science Center, Shantou University, 515063, Shantou, China

    Dinghui Zou & Kunshan Gao

  2. Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China

    Kunshan Gao

Authors
  1. Dinghui Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kunshan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kunshan Gao.

About this article

Cite this article

Zou, D., Gao, K. Photosynthetic bicarbonate utilization inPorphyra haitanensis (Bangiales, Rhodophyta). Chin.Sci.Bull. 47, 1629–1633 (2002). https://doi.org/10.1007/BF03184112

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03184112

Keywords

Search

Navigation