Abstract
The acid tolerance is considered as one of the most important properties of lactic acid bacteria as probiotics. H+-ATPase is one of the genes associated with the ability of acid tolerance. We have cloned and sequenced full length cDNA of γ subunit of H+-ATPase gene inLactobacillus acidophilus MG2-9. The cDNA sequence consists of 975 nucleotides; the putative protein has 320 amino acids.
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Ahrne S., Nobaek S., Jeppsson B., Adlerberth I., Wold A.E., Molin G. (1998). The normalLactobacillus flora of healthy human rectal and oral mucosa. J. Appl. Microbiol., 85 (1): 88–94.
Bengmark S. (1998). Ecological control of the gastrointestinal tract. The role of probiotic flora. Gut, 42: 2–7.
Cotter P.D., Hill C. (2003). Surviving the acid test: responses of Gram-positive bacteria to low pH. Microbiol. Mol. Biol. Rev., 67: 429–453.
Futai M., Kanazawa H. (1983). Structure and function of proton-translocating adenosine triphosphatase (F1F0): biochemical and molecular biological approaches. Microbiol. Rev., 47: 285–312.
Kashket E.R. (1987). Bioenergetics of lactic acid bacteria: cytoplasmic pH and osmotolerance. FEMS Microbiol. Rev., 46: 233–244.
Kimura K., McCartney A.L., McConnell M.A., Tannock G.W. (1997). Analysis of faecal populations of bifidobacteria and lactobacilli and investigation of the immunological responses of their human hosts to the predominant strains. Appl. Environ. Microbiol., 63: 3394–3398.
Kobayashi H., Murakami N. (1982). Regulation of the cytoplasmic pH inStreptococcus faecalis. J. Biol. Chem., 257: 13246–13252.
Kobayashi H., Suzuki T., Kinoshita N., Unemoto T. (1984). Amplification of theStreptococcus faecalis proton-translocating ATPase by a decrease in cytoplasmic pH. J. Bacteriol., 158: 1157–1160.
Kobayashi H. (1985). A proton-translocating ATPase regulates pH of the bacterial cytoplasm. J. Biol. Chem., 260: 72–76.
Kobayashi H., Suzuki T., Unemoto T. (1986). Streptococcal cytoplasmic pH is regulated by changes in the amount and activity of a proton-translocating ATPase. J. Biol. Chem., 261: 627–630.
Koebmann B.J., Nilsson D., Kuipers O.P., Jensen P.R. (2000). The membrane-bound H+-ATPase complex is essential for growth ofLactococcus lactis. J. Bacteriol., 182 (17): 4738–4743.
Marteau P., Rambaud J.C. (1993). Potential of using lactic acid bacteria for therapy and immunomodulation in man. FEMS. Microbiol. Rev., 12: 207–220.
Norin K.E., Persson A.K., Saxerholt H., Midtvedt T. (1991). Establishment ofLactobacillus andBifidobacterium species in germ free mice and their influence on some microflora-associated characteristics. Appl. Environ. Microbiol., 57: 1850–1852.
Savage D.C. (1977). Microbial ecology of the gastrointestinal tract. Ann. Rev. Microbiol., 31: 107–133.
Suzuki T., Kobayashi H. (1989). Regulation of the cytoplasmic pH by a proton-translocating ATPase inStreptococcus faecalis (faecium). A computer simulation. Eur. J. Biochem., 180 (2): 467–471.
Tannock G.W. (1995). Normal Microflora, Chapman & Hall, London.
Wang L., Xu J., Yun Y., Wu R., Menghebilige Zhang H. (2005). Assessment of potential probiotic properties ofLactobacillus isolated from traditionally home-made koumiss in Mongolia. China Dairy Industry, 33 (4): 4–10.
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Chen, X., Sun, Z., Meng, H. et al. Molecular cloning and characterisation of gamma subunit of H+-ATPase inLactobacillus acidophilus MG2-9. Ann. Microbiol. 57, 415–418 (2007). https://doi.org/10.1007/BF03175082
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DOI: https://doi.org/10.1007/BF03175082