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Chiral separation of β-blockers after derivatization with a new chiral derivatization agent, GATC

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Abstract

A new chiral derivatization agent with sugar moiety, 2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl isothiocyanate (GATC) was synthesized. Several β-blockers were investigated for the possible separation of the enantiomers by reversed-phase HPLC after derivatization with this new chiral derivatization agent (GATC). GATC was reacted readily with β-blockers at room temperature and the reaction mixture could directly be injected into the HPLC system. The corresponding diastereomers were well resolved on an ODS column with acetonitrile-ammonium acetate buffer as a mobile phase and monitored at UV 254 nm. The optimization of the derivatiozation procedure (concentration of GATC, reaction temperature and time) and HPLC conditions (pH and ionic strength of mobile phase) were investigated and compared with GITC.

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Authors and Affiliations

  1. College of Pharmacy, Kangwon National University, 200-701, Chunchon, Kangwon, Korea

    Mi Young Ko, Dae Hong Shin, Joung Weon Oh, Workaferhaw Shibru Asegahegn & Kyeong Ho Kim

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  1. Mi Young Ko
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  2. Dae Hong Shin
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  3. Joung Weon Oh
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  4. Workaferhaw Shibru Asegahegn
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  5. Kyeong Ho Kim
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Correspondence to Kyeong Ho Kim.

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Ko, M.Y., Shin, D.H., Oh, J.W. et al. Chiral separation of β-blockers after derivatization with a new chiral derivatization agent, GATC. Arch Pharm Res 29, 1061–1065 (2006). https://doi.org/10.1007/BF02969292

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  • DOI: https://doi.org/10.1007/BF02969292

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