Skip to main content
SpringerLink
Log in

Malate dehydrogenase isozymes as markers of the organelles physiological state in sugar beet (Beta vulgaris L.)

  • Research Article
  • Published:
Sugar Tech Aims and scope Submit manuscript

Abstract

Subcellular localization of NAD-dependent malate dehydrogenase (MDH) isozymes from sugar beet was studied with an integrative method combining differential sedimentation, isopicnic centrifugation in sucrose density and 14% starch gel electrophoresis. Thess andll isozymes, controlled respectively by theMdh2 andMdh3 loci, were found to be located in the mitochondria of young leaves, and thepp isozyme, which is under control of theMdh1 locus, was present in the microbodies. All the assayed MDH isozymes of older leaves were detected in the cytoplasm and in traced amounts in mitochondria. The use of NAD-dependent MDH isozymes for the assessment of subcellular organelles physiological state is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Cammaerts, D. andJacobs, M. (1975). Study of the intracellular location and the genetic control of malate dehydrogenase isozymes inArabidopsis thaliana. Plant Sci. Lett.,4(4): 249–256.

    Article  CAS  Google Scholar 

  • Goodwin, T.W., and Merser, E. I. (1986).Introduction to plant biochemistry. Moscow: Mir. pp. 170–181. (In Russian).

  • Gottlieb, L.D. (1982). Conservation and duplication of isozymes in plants.Science,216(4544): 373–380.

    Article  CAS  Google Scholar 

  • Ikuma, H. (1977). Necessary conditions for isolation of tightly coupled higher plant mitochondria.Plant Physiol.,45(6): 773–781.

    Article  Google Scholar 

  • Korochkin, L.I., Serov, O.L. andManchenko, G.P. (1977). The concept of isozymes. pp. 5–17. In: L.I. Korochkin, O.L. Serov (eds).Genetics of isozymes. Nauka. Moscow. Russia.

    Google Scholar 

  • Levites, E.V., Gorenstein, N.M., Denisova, F.Sh. andTarasova, R.S. (1991). Isoenzymes as markers of genomic instability in sugar beet.Genetika,27(11): 1937–1954 (in Russian).

    CAS  Google Scholar 

  • Levites, E.V., Yudina, R.S. andMaletskii, S.I. (1980). Genetic control of NAD-dependent malate dehydrogenase in sugar beet (Beta vulgaris L.).Dokl. Akad. Nauk SSSR,255(4): 989–991 (in Russian).

    CAS  Google Scholar 

  • Mashansky, V.F., Bobrova, I.F., Drozdov, A.L. andRizhamadze, N.L. (1975). Conservatism in the ultra-structure of mitochondrial membrane components. pp. 345–347. In: A.S. Troshin (ed.)Structure and function of biological membranes. Nauka. Moscow. Russia.

    Google Scholar 

  • Meizel, S. andMarkert, C.L. (1967). Malate dehydrogenase isozymes of the marine snailIlyanassa obsoletes.Arch. Biochem. Biophys.,122(3): 753–765.

    Article  CAS  Google Scholar 

  • Ohnishi, O. (1990). Analysis of genetic variants in common buckwheat,Fagopyrum esculentum Moench. A review,Fagopyrum,10: 1–158.

    Google Scholar 

  • Scandalios, J.G. (1974). Isoenzymes in development and differentiation.Ann. Rev. Plant Physiol.,25: 225–258.

    Article  CAS  Google Scholar 

  • Serov, O.L. (1977). Mechanisms of hybrid isozyme formation as a model for studying gene interaction, pp. 187–197. In: L.I. Korochkin, O.L. Serov (eds).Genetics of isozymes. Nauka. Moscow, Russia.

    Google Scholar 

  • Tarasova, R.S. (1987). Genetically determined forms of NAD-dependent malate dehydrogenase and using them in sugar beet biochemical passportisation.Russian Journal of Genetics,23(9): 1630–1636.

    CAS  Google Scholar 

  • Tarasova, R.S. (1988).Genetics and phenogenetics of sugar beet malate dehydrogenase. Ph.D Thesis, Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

    Google Scholar 

  • Walk, R.-A. andHock, B. (1977). Glyoxysomal and mitochondrial malate dehydrogenase of watermelon (Citrullus vulgaris) cotyledon. II. Kinetic properties of the purified isoenzymes.Planta,136: 221–228.

    Article  CAS  Google Scholar 

  • Walk, R.-A., Michaeli, S. andHock, B. (1977). Glyoxysomal and mitochondrial malate dehydrogenase of watermelon (Citrullus vulgaris) cotyledons. I Molecular properties of the purified isoenzymes.Planta,136: 211–220.

    Article  CAS  Google Scholar 

  • Yamazaki, R.K. andTolbert, N.E. (1969). Malate dehydrogenase in leaf peroxisomes.Biochem. Biophys. Acta,178(1): 11–20.

    CAS  PubMed  Google Scholar 

  • Yudina, R.S., Zheleznova, N.B., Zakharowa, O.V., Zheleznov, A.V., andShumny, V.K. (2005). Isozyme analysis in a genetic collection of amaranths (Amaranthus L.).Russian Journal of Genetics,41(12): 1681–1687.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Cytology and Genetics, SB RAS, 10 Lavrentiev ave., 630090, Novosibirsk, Russia

    Rimma S. Yudina & Evgenii V. Levites

Authors
  1. Rimma S. Yudina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Evgenii V. Levites
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Evgenii V. Levites.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Yudina, R.S., Levites, E.V. Malate dehydrogenase isozymes as markers of the organelles physiological state in sugar beet (Beta vulgaris L.). Sugar Tech 9, 67–71 (2007). https://doi.org/10.1007/BF02956916

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02956916

Keywords

Search

Navigation