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Removal of inhibition by ammonium ion in nitrogenase-dependent hydrogen evolution of a marine photosynthetic bacterium,Rhodopseudomonas sp. strain W-1S

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Abstract

The addition of NH4Cl at concentrations of more than 1 mM completely inhibited nitrogenase-dependent hydrogen evolution using 1 mM succinate as a substrate in the marine photosynthetic bacteriumRhodopseudomonas sp. strain W-1S. However, cells could derepress nitrogenase within 6 h in the presence of NH4Cl. The inhibition by 1 mM NH4Cl was removed by increasing the concentration of the substrate for hydrogen evolution. The addition ofL-methionine-D,L-sulfoximine (MSX), an inhibitor of glutamine synthetase (GS), also removed the inhibition by 1 mM NH4Cl. These results indicated that under an argon gas phase, nitrogenase-dependent hydrogen evolution is inhibited by ATP-consuming GS activity in the presence of NH4Cl. We isolated a mutant strain, CR-8, which is capable of active hydrogen evolution in the presence of 1 mM NH4Cl.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita, 565, Osaka, Japan

    Kiyohito Yagi, Isamu Maeda, Kenji Idehara & Yoshiharu Miura

  2. The Kansai Electric Power Company, Inc., Amagasaki, Hyogo, Japan

    Tohru Akano & Klyomi Fukatu

  3. Mitsubishi Heavy Industries, Ltd., Arai-cho, Hyogo, Japan

    Yoshiaki Ikuta & Hlroshi K. Nakamura

Authors
  1. Kiyohito Yagi
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  2. Isamu Maeda
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  3. Kenji Idehara
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  4. Yoshiharu Miura
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  5. Tohru Akano
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  6. Klyomi Fukatu
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  7. Yoshiaki Ikuta
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  8. Hlroshi K. Nakamura
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Yagi, K., Maeda, I., Idehara, K. et al. Removal of inhibition by ammonium ion in nitrogenase-dependent hydrogen evolution of a marine photosynthetic bacterium,Rhodopseudomonas sp. strain W-1S. Appl Biochem Biotechnol 45, 429–436 (1994). https://doi.org/10.1007/BF02941817

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  • DOI: https://doi.org/10.1007/BF02941817

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