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Peculiarities of the regulation of fermentation and respiration in the crabtree-negative, xylose-fermenting yeastPichia stipitis

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Abstract

The respiration ofPichia stipitis was not repressed by either high concentrations of fermentable sugars or oxygen limitation. Fermentation was not induced by high sugar concentrations, but was inactivated by aerobic conditions. The activity of pyruvate dehydrogenase was constitutive. In contrast, pyruvate decarboxylase, alcohol dehydrogenase, and aldehyde dehydrogenase were induced by a reduction in the oxygen tension. It was demonstrated that inP. stipitis, the pyruvate decarboxylase is not induced by a signal from glycolysis. Contrary toSaccharomyces cerevisiae, the pyruvate decarboxylase was not inhibited by phosphate.

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Authors and Affiliations

  1. Institut für Biologie IV (Mikrobiologie), RWTH Aachen, Worringer Weg, 52074, Aachen, Germany

    Volkmar Passoth, Martin Zimmermann & Ulrich Klinner

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  1. Volkmar Passoth
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  2. Martin Zimmermann
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  3. Ulrich Klinner
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Passoth, V., Zimmermann, M. & Klinner, U. Peculiarities of the regulation of fermentation and respiration in the crabtree-negative, xylose-fermenting yeastPichia stipitis . Appl Biochem Biotechnol 57, 201–212 (1996). https://doi.org/10.1007/BF02941701

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