Abstract
Five mm sections excised from 20, 30 and 40 mm long coleoptiles were transferred into a indole-3-acetic acid (IAA) solution after an incubation in a medium containing buffer and sucrose without IAA. The ability of sections to elongate in dependence on IAA treatment was rapidly decreased at a temperature of 23 °C within a period of time depending both on the duration of incubation and on the age of coleoptiles from which sections were obtained. Under the same conditions of incubation and age the same decrease of the ability of sections to elongate at 4 °C was, however, observed only after a period fourfold longer than at 23 °C. Incubation of sections in the presence of benzimidazole did not extend their reactivity to IAA. This phenomenon may be therefore considered as a senescence reaction which is more effective at 23 °C than at 4 °C.
Abstract
Pětimilimetrové segmenty odebírané z koleoptili dlouhých 20, 30 a 40 mm byly indukovány v mediu bez indolyl-3-octové kyseliny (IAA) obsahujícím pufr a sacharosu a později přeneseny do roztoku IAA. Při 23 °C segmenty rychle ztrácely schopnost prodlužování indukovaného IAA během časového údobí, jehož délka závisela na délce inkubace a stář í koleptilí z nichž byly segmenty odděleny, Při 4 °C byla však ztráta schopnosti prodlužování čtyřikrát pomalejší při stejných podmínkách kultivace a stejném staří. Inkubace za přítomnosti benzilimidazolu neprodlužovala citlivost na IAA. Tento jev můžeme proto považovat za reakei stárnuti, probíhající rychleji př i 23 °C než při 4 °C.
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Fantinet, M. Sénescence de segments de coleoptiles d’avoine et élongation due à l’auxine. Biol Plant 14, 286–292 (1972). https://doi.org/10.1007/BF02935854
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DOI: https://doi.org/10.1007/BF02935854