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Effective extraction of oligomeric proanthocyanidin (OPC) from wild grape seeds

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Abstract

The Oligomeric proanthocyanidin (OPC) in green and black tea, grape seeds, grapes and wine has raised much attention but that OPC in wild grape seed remains to be intensively investigated. This study investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process with various temperatures, solvent compositions and times. Also, a chromatography column packed with the Dia-ion HP-20 resin was used for further purification of the OPC. The total OPC contents were determined with the Folin-Ciocalteu reagent, and the antioxidant activity using total antioxidant potential (TAP) and 1,1-diphenyl-2picrylhydrazyl (DPPH). The yield of final purified OPC was 1.78 (+)-catechin equivalent (CE) g/100 g, with IC50 activities of TAP and DPPH of 31.60 and 15.70 μg/mL. These activities of the final purified OPC were about two times higher than that of the BHA used as a reference sample.

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References

  1. de Gaulejac, C. T., Y. Glories, and N. Vivas (1999) Analysis of anthocaynidins in foods by liquid chromatography, liquid chromatography-mass spectrometry and capillary electrophoresis.J. Chromatogr. A 881: 327–333.

    Google Scholar 

  2. de Freitas, V. A. P., Y. Glories, G. Bourgeois, and C. Vitry (1998) Characterization of oligomeric and polymeric proanthocyanidins from grape seeds by liquid second ion mass spectrometry.Phytochemistry 49: 1435–1441.

    Article  Google Scholar 

  3. Pekic, B., V. Kovac, E. Alonso, and E. Revilla (1998) Study of the extraction of proanthocyanidins from grape seeds.Food Chem. 61: 201–206.

    Article  CAS  Google Scholar 

  4. Catapano, A. L. (1997) Antioxidant effect of flavonoids.Angiology, 48: 39–44.

    Article  CAS  Google Scholar 

  5. Hagerman, A. E., K. M. Riedl, G. A. Jones, K. N. Sovik, N. T. Ritchard, P. W. Hartzfeld, and T. L. Riechel (1998) High molecular weight plant phenolics (tanins) as biological antioxidant.J. Agric. Food Chem. 46: 1887–1892.

    Article  CAS  Google Scholar 

  6. Bravo, L. (1998) Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance.Nutr. Rev. 56: 317–333.

    Article  CAS  Google Scholar 

  7. Fuleki, T. J. and M. R. da Silva (1997) Catechin and proanthocyanidin composition of seeds from grape cultivars grown in Ontario.J. Agric. Food Chem. 45: 1156–1160.

    Article  CAS  Google Scholar 

  8. Santos-Buelga, C., E. M. Francia-Aricha, and M. T. Escribano-Bailon (1995) Comparative falvan-3-ol composition of seeds from different grape varieties.Food Chem. 53: 197–201.

    Article  CAS  Google Scholar 

  9. Prakash, G., S. S. Bhojwani, and A. K. Srivastava (2002) Production of azadirachtin from plant tissue culture: State of the art and future prospects.Biotechnol. Bioprocess Eng. 7: 185–193.

    Article  CAS  Google Scholar 

  10. Yamakoshi, J., M. Satio, S. Kataoka, and M. Kikuchi (2002) Safety evaluation of proanthocyanidin-rich extract from grape seeds.Food Chem. Toxicol. 40: 599–607.

    Article  CAS  Google Scholar 

  11. Bagchi, D., R. L. Krohn, A. Garg, J. Balmoori, M. Bagchi, D. J. Bagchi, M. X. Tran, and S. J. Stohs (1997) Comparativein vitro andin vivo free radical scavenging abilities of grape seed proanthocyanidins and selected antioxidants.FASEB J. Abstr. 11: 3369.

    Google Scholar 

  12. Kim, M. A. and J. K. Park (2002) High frequency plant regeneration of garlic (Allium sativum L.) calli immobilized in calcium alniante gel.Biotechnol. Bioprocess Eng. 7: 206–211.

    Article  CAS  Google Scholar 

  13. Frankel, E. N., A. L. Waterhouse, and P. L. Teissedre (1995) Principal phenolic phytochemicals in selected California wines and their antioxidant activity in inhibiting oxidation of human low-density lipoproteins.J. Agric. Food Chem. 43: 890–894.

    Article  CAS  Google Scholar 

  14. Campodonico, P., E. Barbieri, M. Pizarro, C. P. Sotomayor, and E. A. Lissi (1998) A comparison between total phenol content of wines and their TRAP values measured by the bleaching of ABTS radical cations.B. Soc. Chil. Quim. 43: 281–285.

    CAS  Google Scholar 

  15. Chen, C. W. and C. T. Ho (1995) Antioxidant properties of poly phenols extracted from green and black tea.J. Food Lipids 2: 35–46.

    Article  CAS  Google Scholar 

  16. Singleton, V. L., R. Orthofer, and R. M. Lamuela-Raventos (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteau reagent.Method Enzymol. 299: 152–178.

    Article  CAS  Google Scholar 

  17. Sun, B., C. Leandro, J. R. da Silva, and I. Spranger (1998) Separation of grape and wine proanthocyanidins according to their degree of polymerization.J. Agric. Food Chem. 46: 1390–1396.

    Article  CAS  Google Scholar 

  18. Chen, C. C., L. L. Shi, and C. C. Chen (1996) Effect of extraction temperature and time on polyphenol contents and composition and sensory quality of oolong tea infusion.Food Sci. Taiwan 23: 285–298.

    CAS  Google Scholar 

  19. Robinson, E. E., S. R. J. Maxwell, and G. H. G. Thorpe (1997) An investigation of the antioxidant activity of black tea using enhanced chemiluminescence.Free Radical Res. 26: 291–302.

    Article  CAS  Google Scholar 

  20. Nonaka, G. I., N. Miwa, and I. Nishioka (1982) Stilbene glycoside gallates and proanthocyanidins from polygomum multiflorum.Phytochemistry 21: 429–432.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Korea

    Yun Suk Huh & Won Hi Hong

  2. Department of Food & Nutrition, Daejeon Health Science College, 300-711, Daejeon, Korea

    Tae Hee Hong

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  1. Yun Suk Huh
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  2. Tae Hee Hong
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  3. Won Hi Hong
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Correspondence to Won Hi Hong.

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Huh, Y.S., Hong, T.H. & Hong, W.H. Effective extraction of oligomeric proanthocyanidin (OPC) from wild grape seeds. Biotechnol. Bioprocess Eng. 9, 471–475 (2004). https://doi.org/10.1007/BF02933488

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  • DOI: https://doi.org/10.1007/BF02933488

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