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α5β1 integrins and fibronectin are involved in adherence of thePseudomonas aeruginosa ER97314 clinical strain to A549 cells

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Abstract

The role of fibronectin (Fn) and its natural receptors α5β1 integrins in the interaction ofP. aeruginosa with A549 epithelial cells was compared in the clinical isolate ER97314 and the reference PAK strain. Both strains expressed functional type IV pili, as shown by the results of the twitching motility assay. The ER97314 strain was highly adherent to immobilized Fn (640 000 ± 20 000 CFU per well) while the PAK strain adhered less efficiently (70 000 ± 10 000 CFU per well). Both strains adhered to A549 cells (33 400 ± 1200 and 1200 ± 100 CFU per well, for PAK and ER97314, respectively), only the PAK strain being significantly internalized (9430 ± 2020 CFU per well). Cytochalasin D and genistein significantly decreased bacterial adherence of the 2 strains and caused also a significant decrease in PAK internalization. This inhibitory activity was not related to changes in the expression of α5β1 integrins. Antibodies to Fn and α5β1 integrins inhibited the adherence of the ER97314 strain but had no significant effect on PAK interaction with human cells. These findings suggest that only someP. aeruginosa strains can target Fn and their natural receptors α5β1 integrins for adherence to A549 cells.

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Abbreviations

BSA:

bovine serum albumin

Fn:

fibronectin

MOI:

multiplicity of infection

PTK:

protein-tyrosine kinases (EC 2.7.1.112)

ECM:

extracellular matrix

Mabs:

monoclonal antibodies

PBS:

phosphate-buffered saline

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Author notes

  1. H. Gagniěre

    Present address: Ecole de Biologie Industrielle, Cergy, France

Authors and Affiliations

  1. Laboratoire ERRMECe EAI391, Université de Gergy-Pontoise, 95302, Cedex, France

    H. Gagniěre & P. Di Martino

Authors
  1. H. Gagniěre
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  2. P. Di Martino
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Correspondence to P. Di Martino.

Additional information

This work was supported by theMinistère de la Recherche et de l’Enseignement Supérieur.

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Gagniěre, H., Di Martino, P. α5β1 integrins and fibronectin are involved in adherence of thePseudomonas aeruginosa ER97314 clinical strain to A549 cells. Folia Microbiol 49, 757–762 (2004). https://doi.org/10.1007/BF02931561

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  • DOI: https://doi.org/10.1007/BF02931561

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