Abstract
GATA-rich Bkm-related DNA in the mouse Y chromosome is closely associated with the sex determining locus in the Sxr (sex reversed) mutation (Singh L and Jones K W,Cell 28: 205–216, 1982). Bkm-related male-specific DNA was gel separated from male mouse DNA as a > 23 kb band afterHue III andBst NI digestions and microinjected into mouse zygotes to determine its effects on sexual differentiation. We observed that 1 out of 7 XX embryos and 3 out of 35 weaned female mice possessed a > 23 kb male-specific band, though the hybridization intensity was very low in comparison to the normal control male. Two presumptive male carriers of the injected Bkm DNA were identified by progeny tests. Various sporadic anomalies in sexual phenotypes were noted but no obvious connection was established between these and the injected Bkm-related DNA. No significant effects on sex ratio were found.
Similar content being viewed by others
References
Beechey C V, Kirk M and Searle A G 1980 Reciprocal translocation induced in an oocyte and affecting fertility in male mice.Cytogenet. Cell Genet. 27: 129–146
Biggers J D, Whitten W K and Whittingham D G 1971 The culture of mouse embryosin vitro. InMethods in mammalian embryology (ed.) J C Daniel Jr (Freeman: San Francisco) pp. 86–116
Bittner M, Kupferer P and Morris C F 1980 Electrophoretic transfer of proteins and nucleic acids from slab gels to diazobenzyloxymethyl cellulose or nitrocellulose sheets.Anal. Biochem. 102: 459–471
Bucchini D, Ripoche M A, Stinnakre M G, Desbois P, Lores P, Monthioux E, Absil J, Lepesant J A, Pictet R and Jami J 1986 Pancreatic expression of human insulin gene in transgenic mice.Proc. Nail. Acad. Sci., USA 83: 2511–2515
deBoer P and Searle A G 1980 Chromosomal aspects of male sterility.J. Reprod. Ferlil. 60: 259–265
Fisher E M C, Cavanna J S and Brown S D M 1985 Microdissection and microcloning of the mouse X chromosome.Proc. Nail. Acad. Sci., USA 82: 5846–5849
Gordon J W, Scangos G A, Plotkin D J, Barbosa J A and Ruddle F H 1980 Genetic transformation of mouse embryos by microinjection of purified DNA.Proc. Natl. Acad. Sci., USA 77: 7380–7384
Grosschedl R, Weaver D, Baltimore D and Costantini F 1984 Introduction of an immunoglobulin gene into the mouse germ line: Specific expression in lymphoid cells and synthesis of functional antibody.Cell 38: 647–658
Guellaen G, Casanova M, Bishop C, Geldwerth D, Andre G, Fellous M and Weisscnbach J 1984 Human XX males with Y single-copy DNA fragments.Nature (itLondon) 307: 172–173
Hu N and Messing J 1982 The making of strand-specific M13 probes.Gene 17: 271–277
Lyon M F 1963 Attempt to test the inactive-X theory of dosage compensation in mammals.Genet. Res. 4: 93–103
Maniatis T, Kee S G, Efstratiadis A and Kafatos F C 1976 Amplification and characterization of a globin gene synthesizedin vitro.Cell 8: 163–182
McLaren A, Simpson E, Tomonari K, Chandler P and Hogg H 1984 Male sexual differentiation in mice lacking H-Y antigen.Nature (London) 312: 552–555.
Palmiter R D, Chen H Y and Brinster R L 1982 Differential regulation of methallothionein-thymidine kinase fusion genes in transgenic mice and their offspring.Cell 29: 701–710
Palmiter R D, Wilkie T M, Chen H Y and Brinster R L 1984 Transmission distortion and mosaicism in an unusual transgenic mouse pedigree.Cell 36: 869–877
Rohme D, Fox H, Herrmann B, Frischauf A M, Edstrom J E, Mains P, Silver L M and Lehrach H 1984 Molecular clones of the mouse t complex derived from microdissected metaphase chromosomes.Cell 36: 783–788
Scalenghe F, Turco E, Edstrom J E, Pirrotta V and Melli M 1981 Microdissection and cloning of DNA from a specific region ofDrosophila melanogaster polytene chromosomes.Chromosoma 82: 205–216
Selden R F, Skoskiewicz M J, Howie K B, Russell P S and Goodmann H M 1986 Regulation of human insulin gene expression in transgenic mice.Nature (London) 321: 525–528
Singh L and Jones K W 1982 Sex reversal in the mouse (Mus musculus) is caused by a recurrent nonreciprocal crossover involving the X and an aberrant Y chromosome.Cell 28: 205–216
Singh L and Jones K W 1984 The use of heparin as a simple cost-effective means of controlling background in nucleic acid hybridization procedures.Nucleic Acid Res. 12: 5627–5638
Singh L and Jones K W 1986 Bkm sequences are polymorphic in humans and are clustered in pcricentric legions of various acrocentric chromosomes including the Y.Hum. Genet. 73: 304–308
Singh L, Phillips C and Jones K W 1984 The conserved nucleotide sequences of Bkm, which defineser in the mouse, are transcribed.Cell 35: 111–120
Singh L, Purdom I F and Jones K W 1981 Sex-chromosome-associated nucleotide sequences in eukaryotes.Cold Spring Harbor Symp. Quant. Biol. 45: 805–813
Singh L, Matsukuma S and Jones K W 1987 Testis development in a mouse with 10% of XY cells.Dev. Biol. 122: 287–290
Southern E M 1975 Long range periodicities in mouse satellite DNA.J. Mol. Biol. 94: 51–69
Swift G H, Hammer R E, MacDonald R J and Brinster R L 1984 Tissue-specific expression of the rat pancreatic elastase I gene in transgenic mice.Cell 38: 639–646
Vergnaud G, Page D C, Simmler M-C, Brown L, Rouyer F, Noel B, Botstein A, de la Chapeile A and Weisscnbach J 1986 A deletion map of the human Y chromosome based on DNA hybridization.Am. J. Hum. Genet. 38: 109–124
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matsukuma, S., Singh, L. & Jones, K.W. Microinjection of Bkm-related male-specific mouse DNA into autologous zygotes. J. Genet. 66, 163–176 (1987). https://doi.org/10.1007/BF02927710
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02927710