Skip to main content
SpringerLink
Log in

Reactivity of alkaline protease to keratin and collagen containing substances

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The reactivity of partially purified alkaline protease fromBacillus subtilis, to keratin and collagen containing substances has been investigated. The experimentally obtained apparent values of the Michaelis-Menten constant (Km), the maximum reaction rate (Kmax, and the energy of activation (Ea), lead to the conclusion that:

  1. 1.

    The keratin containing substrates are not so easily digested as those containing collagen.

  2. 2.

    The kinetics of the reaction with time has been investigated.

The relationship of Kmax was a function of temperature, aiming at the industrial application of this reaction, has been obtained.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Svedsen, I. (1976),Carlsberg Res. Commun. 41, 237–291.

    Article  Google Scholar 

  2. Morihara, T., Oka, T., and Tzuzuki, H. (1974),Arch. Biochem. Biophys. 165, 7–79.

    Article  Google Scholar 

  3. Nedkov, P., Oberthur, W., and Braunitzer, G. (1985),Hoppe-Seyler’s Z. Physiol. Chem. 366, 421–430.

    CAS  Google Scholar 

  4. Schester, I. and Berger, A. (1967),Biochem. Biophys. Res. Commun. 7, 157–162.

    Article  Google Scholar 

  5. Bode, W., Papamakos, E., and Musil, U. (1986),EMBO J. 3, 813–818.

    Google Scholar 

  6. McPhalen, C, Schneibli, H., and James, M. (1985),FEBS Lett. 18, 55–58.

    Article  Google Scholar 

  7. Boteva, R. and Dimov, I. (1988),J. Photochem. Photobiol. B: Biology 1, 461–468.

    Article  CAS  Google Scholar 

  8. Toushek, D., Nedkov, P., Genov, N., and Shopova, M. (1983),Commun. Dept. Chem. Bulg. Acad. Sci. 16, 443–449.

    Google Scholar 

  9. Möberg, U. and Ferenström, H. (1977), LKB Application Note 306.

  10. Winter, A. and Anderson, U. (1977), LKB Application Note 50.

  11. Nedkov, P., Bobatinov, M., Shopova, M., and Genov, N. (1976),Commun. Dept. Chem. Bulg. Acad. Sci. 9, 443–449.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, University of Sofia, 8 Dragan Zankov Str., 1421, Sofia, Bulgaria

    P. Dalev

  2. Central Laboratory of Biophysics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    V. Neitchev

Authors
  1. P. Dalev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Neitchev
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dalev, P., Neitchev, V. Reactivity of alkaline protease to keratin and collagen containing substances. Appl Biochem Biotechnol 27, 131–138 (1991). https://doi.org/10.1007/BF02921521

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02921521

Index Entries

Search

Navigation