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The stimulatory effect of uracil and 5-bromouracil on the seed set inPapaver somniferum L.

Stimulaění vliv uracilu a 5-bromuracilu na násadu semen uPapaver somniferum L.

  • Published:
Biologia Plantarum

Abstract

A study was made concerning the effect of the analogues of nucleic acid components upon the seed set inPapaver somniferum L. For the experiments flowers one day after anthesis were used, as the pollination taking place at this flower age had previously been found to be most efficient for the seed set. The solutions of analogues in a 10 per cent sucrose were applied by injecting them into the ovaries three hours prior to pollination. Damage to the ovary by the injection reduces the seed set by about two thirds.

In comparison with control flowers, into which only a sucrose solution was injected, 5-bromouracil at a concentration of 5.2×10−4 M increases the seed set by about 70 per cent. Uracil brings about the same degree of promotion. The same concentration of 5-bromodeoxyuridine has no significant effect on the seed set. When applied simultaneously with uracil it suppresses its stimulating effect completely. The equimolar mixture of uracil and bromouracil did not cause any significant increase in the number of seeds in the capsules, either.

Abstract

Bylo sledováno ovlivnění násady semen analogy složek nukleových kyselin. K pokusům byly používány květy jeden den před anthesis, když bylo před tím zjištěno, že v tomto stáří opylované květy dávají nejvyšší násadu semen. Roztoky analog v 10% sacharose byly aplikovány injekeí do semeníků tři hodiny před opylením. Vlivem poškození semeníku injekeí roztoku dochází ke snížení násady semen zhruba o dvě třetiny.

Ve srovnání s kontrolními květy, do nichž byl injikován pouze roztok sacharosy, zvyšuje 5-bromuracil v koncentraci 5.2×10−4 M násadu semen přibližně o 70%. Stejné zvýšení způsobuje uracil. 5-bromdeoxyuridin v téže koncentraci násadu semen průkazně neovlivňuje.

Je-li aplikován současně s uracilem, zeela potlačuje jeho stimulační účinek. Rovněž ekvimolární směs uracilu s bromuracilem nezpůsobila průkazné zvýšení počtu semen v tobolkách.

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Address: Flemingovo nám. 2, Praha 6, Czechoslovakia.

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Balatková, V., Tupý, J. The stimulatory effect of uracil and 5-bromouracil on the seed set inPapaver somniferum L.. Biol Plant 14, 140–145 (1972). https://doi.org/10.1007/BF02920958

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