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Neuroendocrine neoplasms of the lung are not associated with point mutations at codon 12 of the Ki-ras gene

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Virchows Archiv B

Summary

The most prominent abnormality ofras proto-oncogenes in human lung tumours has involved point mutations at codon 12 of the Ki-ras gene. We have analysed 35 tumour samples of neuroendocrine lung neoplasms (ten carcinoid tumours, ten well-differentiated neuroendocrine carcinomas, and 15 intermediate/small cell neuroendocrine carcinomas) for a point mutation at this site. For this purpose, formalin-fixed and paraffin-embedded tissue sections were microdissected to remove non-tumorous areas. DNA in the remaining tumour tissue was amplified in vitro by the polymerase chain reaction (PCR) and double-stranded PCR products were subjected to sequence analysis. Neither point mutations at codon 12 nor additional structural alterations at codons 1–32 were detected in the Ki-ras gene. Our results suggest that point mutations at codon 12 of the Ki-ras gene do not seem to be involved in the pathogenesis of pulmonary neuroendocrine neoplasms.

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Wagner, S.N., Müller, R., Boehm, J. et al. Neuroendocrine neoplasms of the lung are not associated with point mutations at codon 12 of the Ki-ras gene. Virchows Archiv B Cell Pathol 63, 325–329 (1993). https://doi.org/10.1007/BF02899279

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  • DOI: https://doi.org/10.1007/BF02899279

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