Summary
The angiogenesis induced after implantation of fragments of the Walker 256 carcinoma was compared with the angiogenesis following implantation of different amounts of Indian ink. Morphologically and chronologically the tumour system showed no difference from the Indian ink system, provided sufficient amounts of ink were implanted. Both systems were characterized by significant macrophage infiltration. The vascular development, which was clearly concentrated in a dense rim around the tumour, remained present when the tumour enlarged, suggesting an acquisition of vasculature by the tumour through vessel incorporation and not vessel ingrowth. Initially, scattered desmin-positive cells, in contact or encircled by collagen IV, were found in the developing angiogenic rim. Later many desmin-positive cells were found around vessels and could be identified by electron microscopy as pericytes. They exhibited close local contacts with endothelial cells. After incorporation of the peritumour vascular rim into the tumour the number of pericytes decreased and their shape became flattened and elongated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altmannsberger M, Osborn M, Schauer A, Weber K (1981) Antibodies to different intermediate filament proteins. Cell type-specific markers on paraffin-embedded human tissues. Lab Invest 45:427–434
Altmannsberger M, Weber K, Droste R, Osborn M (1985) Desmin is a specific marker for rhabdomyosarcomas of human and rat origin. Am J Pathol 118:85–95
Ausprunk DH, Folkman J (1977) Migration and proliferation of endothelial cells in preformed and newly formed blood vessels during tumor angiogenesis. Microvasc Res 14:53–65
Bär T, Wolff JR (1972) The formation of capillary basement membranes during internal vascularization of the rat’s cerebral cortex. Z Zeilforsch 133:231–248
Broers JLV, Carney DN, Klein Rot M, Schaart G, Lane EB, Vooys GP, Ramaekers FCS (1986) Intermediate filament proteins in classic and variant types of small lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies. J Cell Sci 83:37–60
Cavallo T, Sade R, Folkman J, Cotran RS (1973) Ultrastructural autoradiographic studies of the early vasoproliferative response in tumor angiogenesis. Am J Pathol 70:345–362
Crocker DJ, Murad TM, Geer JC (1970) Role of the pericyte in wound healing. An ultrastructural study. Exp Mol Pathol 13:51–65
Davies PF (1986) Vascular cell interactions with special reference to the pathogenesis of atherosclerosis. Lab Invest 55:5–24
Debus E, Weber K, Osborn M (1983) Monoclonal antibodies to desmin, the muscle-specific intermediate filament protein. EMBO J 2:2305–2312
Folkman J (1976) The vascularization of tumors. Sci Am 234:58–73
Folkman J, Haudenschild C (1980) Angiogenesis in vitro. Nature 288:551–556
Form DM, Pratt BM, Madri JA (1986) Endothelial cell proliferation during angiogenesis. In vitro modulation by basement membrane components. Lab Invest 55:521–530
Fujimoto T, Singer SJ (1986) Immunocytochemical studies of endothelial cells in vivo. I. The presence of desmin only, or of desmin plus vimentin, or vimentin only, in the endothelial cells of different capillaries of the adult chicken. J Cell Biol 103:2775–2786
Furcht LT (1986) Critical factors controlling angiogenesis: cell products, cell matrix and growth factors. Lab Invest 55:505–509
Gabbiani G, Schmid E, Winter S, Chaponnier C, de Chastonay C, Vandekerckhove J, Wever K, Franke WW (1981) Vascular smooth muscle cells differ from other smooth muscle cells: predominance of vimentin filaments and a specific alpha-type actin. Proc Natl Acad Sci USA 78:298–302
Krylova NV (1968) Characteristics of microcirculation in experimental tumours. Bibl Anat 10:301–303
Lazarides E (1980) Intermediate filaments as mechanical integrators of cellular space. Nature 283:249–255
Leader M, Collins M, Patel J, Henry K (1987) Desmin: its value as a marker of muscle derived tumours using a commercial antibody. Virchows Arch [Pathol Anat] 411:345–349
Le Beux YJ, Willemot J (1978) Actinand myosin-like filaments in rat brain pericytes. Anat Rec 190:811–826
Maxwell MH (1977) Two rapid and simple methods used for the removal of resins from 1 μm thick epoxy sections. J Microsc 112:253–255
Nunnery EW, Kahn LB, Reddick RL, Lipper S (1981) Hemangiopericytoma: A light microscopic and ultrastructural study. Cancer 47:906–914
Orlidge A, D’Amore PA (1986) Pericyte and smooth muscle cell modulation of endothelial cell proliferation. J Cell Biol 103, part 2, 471a
Osborn M, Weber K (1983) Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 48:372–394
Polverini PJ, Leibovich SJ (1984) Induction of neovascularization in vivo and endothelial proliferation in vitro by tumorassociated macrophages. Lab Invest 51:635–642
Polverini PJ, Cotran RS, Gimbrone MA, Unanue ER (1977) Activated macrophages induce vascular proliferation. Nature 269:804–806
Ramaekers FCS, Puts JJG, Kant A, Moesker O, Jap PHK, Vooys GP (1982) Use of Antibodies to intermediate filaments in the characterization of human tumors. Cold Spring Harbor Symp Quant Biol 46:331–339
Ramaekers FCS, Verheyen RHM, Moesker O, Kant A, Vooys GP, Herman CJ (1983) Mesodermal mixed tumor. Diagnosis by analysis of intermediate filament proteins. Am J Surg Pathol 7:381–385
Reinhold HS, van den Berg-Blok A (1984) Factors influencing the neovascularization of experimental tumours. Biorheology 21:493–501
Rhodin JAG (1974) Cardiovascular system. In: Histology: a text and atlas. Oxford University Press, New York, pp 331–370
Roholl PJM, de Jong ASH, Ramaekers FCS (1986) Diagnostic markers in soft tissue tumors. In:van Oosterom A, van Unnik J (eds). Management of soft tissue and bone sarcomas. Raven Press, New York, pp 35–64
Rouget C (1873) Mémoire sur le développement, la structure et les propriétés physiologiques des capillaires sanguins et lymphatiques. Arch Physiol Norm Pathol 5:603–663
Schor AM, Schor SL (1983) Tumour angiogenesis. J Pathol 141:385–413
Skalli O, Gabbiani G (1986) The biology of the myofibroblast in relation to wound contraction and fibrocontractive diseases. In: Clark R, Henson P (eds) The molecular and cellular biology of wound repair. Plenum Publ Coop, New York, pp 3–36
Somlyo AP, Somlyo AV (1968) Vascular smooth muscle. I. Normal structure, pathology, biochemistry, and biophysics. Pharmacol Rev 20:197–272
Staubesand J, Andres KH (1953) Grafische Rekonstruktion zur räumlichen Darstellung präterminaler Gefäße und intravasaler Besonderheiten. Mikroskopie 8:111
Sternberger LA (1979) The unlabeled antibody method: Hormone receptor, Golgi-like and dual color immunocytochemistry. J Histochem Cytochem 27:1658–1659
Suzuki M, Hori K, Abe I, Saito S, Sato H (1984) Functional characterization of the microcirculation in tumors. Cancer Metastas Rev 3:115–126
Thompson WD, Shiach KJ, Fraser RA, McIntosh LC, Simpson JG (1987) Tumours acquire their vasculature by vessel incorporation, not vessel ingrowth. J Pathology 151:323–332
Van Cauwenberge D, Pierard GE, Foidart JM, Lapiere CM (1983) Immunohistochemical localization of laminin, type IV and type V collagen in basal cell carcinoma. Br J Dermatol 108:163–170
Warren BA (1970) The ultrastructure of the microcirculation at the advancing edge of Walker 256 carcinoma. Microvasc Res 2:443–453
Warren BA (1979) The vascular morphology of tumors. In: Peterson H-I (ed) Tumor blood circulation. CRC Press, Florida, pp 1–47
Author information
Authors and Affiliations
Additional information
Part of this work was supported by grant nr. 3.0019.85 from the N.F.G.W.O.
Rights and permissions
About this article
Cite this article
Verhoeven, D., Buyssens, N. Desmin-positive stellate cells associated with angiogenesis in a tumour and non-tumour system. Virchows Archiv B Cell Pathol 54, 263–272 (1987). https://doi.org/10.1007/BF02899222
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02899222