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Spezifische Zellfunktionen von Zell- und Gewebekulturen

I. Züchtung von Melanin-bildenden Zellen des Harding-Passey-Melanoms in Monolayer-Kultur

Specific cell functions of cell and tissue cultures

I. Cultivation of melanin-producing cells of the Harding-Passey-melanoma in monolayer culture

  • Published:
Virchows Archiv B

Summary

Cells were isolated from tissue fragments of the Harding-Passey-melanoma of a NMRI-mouse and cultivated in monolayer cell culture since 3 years. These cells have maintained the capability to produce melanin. The culture medium consisted of Eagle’s basal medium supplemented with 10% fetal calf serum. The cells were subcultivated 30 times, so far. The average population doubling-time was about 100 hours.

29 out of 75 inoculated mice developed solid, not-metastasizing, melanotic tumors after subcutaneous injection of culture cells.

Proliferating cell-cultures contained little melanin; however the melanin content strongly increased with increasing cell density and decreasing cell proliferation-rate.

Possible relationships between inhibition of cell multiplication and “initiation” of a specific cell function (melanin-synthesis) were discussed.

Zusammenfassung

Aus Gewebefragmenten eines Harding-Passey-Melanoms einer NMRI-Maus wurden Zellen isoliert und seit 3 Jahren in Monolayer-Zellkultur unter Erhalt der Fähigkeit zur Melaninsynthese weitergezüchtet. Als Nährmedium diente Eagle’s Basal-medium, welches mit 10% fetalem Kalbsserum ergänzt wurde. Die Zellen wurden bisher 30mal subkultiviert. Die durchschnittliche Verdopplungszeit betrug etwa 100 Std.

Nach subcutaner Injektion von Kulturzellen entwickelten sich bei 29 von 75 inoculierten Mäusen solide, nicht-metastasierende, melanotische Tumoren.

Kulturen von proliferierenden Zellen enthielten wenig Melanin; dagegen stieg der Melaningehalt bei zunehmender Zelldichte und verminderter Vermehrungsgeschwindigkeit stark an.

Mögliche Zusammenhänge zwischen Hemmung der Zellvermehrung und Ausprägung einer spezifischen Zellfunktion (Melaninsynthese) wurden diskutiert.

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Literatur

  • Bancroft, F. C., Levine, L., Tashjian, A. H., Jr.: Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone. J. Cell Biol.43, 432–441 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Barban, S.: Differential action of hormones on induced resistance to 2-Deoxy-D-glucose in mammalian cell cultures. Biochim. biophys. Acta (Amst.)115, 197–204 (1966).

    CAS  Google Scholar 

  • Cahn, R. D., Cahn, M. B.: Heritability of cellular differentiation: clonal growth and expression of differentiation in retinal pigment cells in vitro. Proc. nat. Acad. Sci. (Wash.)55, 106–114 (1966).

    Article  CAS  Google Scholar 

  • Castor, C. W.: The rate of hyaluronic acid production by human synovial cells studied in tissue culture. Arthr. and Rheum.2, 259–265 (1959).

    Article  CAS  Google Scholar 

  • Claunch, C., Oikawa, A., Tchen, T. T., Hu, F.: Biochemical studies on certain “pigmented” and “nonpigmented” strains of melanoma cells. In: Advances in biology of skin, vol. VIII, The pigmentary system, p. 479–488. Oxford and New York: Pergamon Press 1967.

    Google Scholar 

  • Cox, R. P., MacLeod, C. M.: Regulation of alkaline phosphatase in human cell cultures. Cold Spr. Harb. Symp. quant. Biol.29, 233–251 (1964).

    CAS  Google Scholar 

  • Doljanski, L.: Sur le rapport entre la prolifération et l’activité pigmentogène dans les cultures d’épithélium de l’iris. C. R. Soc. Biol. (Paris)105, 343–345 (1930).

    Google Scholar 

  • Ebeling, A. H.: Cultures pures d’ épithelium proliférant in vitro depuis dix-huit mois. C. R. Soc. Biol. (Paris)90, 562–563 (1924).

    Google Scholar 

  • Ephrussi, B., Temin, H.M.: Infection of chick iris epithelium with the Rous sarcoma virus in vitro. Virology11, 547–552 (1960).

    Article  PubMed  CAS  Google Scholar 

  • Fischer, I.: Die Pigmentbildung des Irisepithels in vitro. Arch. exp. Zellforsch.21, 92–154 (1938).

    Google Scholar 

  • Fischer, R., Schachtschabel, D., Zilliken, F.: Biochemische Untersuchungen zur Beeinflussung der Melanin-Bildung von Zellkulturen des Harding-Passey-Melanoms. Hoppe-Seylers Z. physiol. Chem.350, 1168 (1969).

    PubMed  CAS  Google Scholar 

  • Foley, G. E., Drolet, B. P., McCarthy, R. E., Goulet, K. A., Dokos, J. M., Filler, D. A.: Isolation and Serial Propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines. Cancer Res.20, 930–939 (1960).

    PubMed  CAS  Google Scholar 

  • Gelehrter, T. D., Tomkins, G. M.: The role of RNA in the hormonal induction of tyrosine aminotransferase in mammalian cells in tissue culture. J. molec. Biol.29, 59–76 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Goldberg, B., Green, H., Todaro, G. J.: Collagen formation in vitro by established mammalian cell lines. Exp. Cell Res.31, 444–447 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Grossfeld, H.: Mechanism of atherogenic action of excess hydrocortisone. Nature (Lond.)184, 38–41 (1959).

    Article  CAS  Google Scholar 

  • Harding, H. E., Passey, R. D.: A transplantable melanoma of the mouse. J. Path. Bact.33, 417–427 (1930).

    Article  Google Scholar 

  • Hirschhorn, K.: Procedure-Micro-cultures for chromosome studies. In: Gibco Price and Refere nce Manual, p. 70. Grand Island Biological Company 1966.

  • Hu, F., Lesney, P. L.: The isolation and cytology of two pigment cell strains from B 16 mouse melanomas. Cancer Res.24, 1634–1643 (1964).

    PubMed  CAS  Google Scholar 

  • Kowal, J.: Metabolic events associated with steroid biosynthesis in adrenal tissue cultures. Trans. N. Y. Acad. Sci.31, 359–378 (1969).

    PubMed  CAS  Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).

    PubMed  CAS  Google Scholar 

  • Merchant, D. J., Kahn, R. H.: Fiber formation in Suspension cultures of L strain fibroblasts. Proc. Soc. exp. Biol. (N.Y.)97, 359–362 (1958).

    CAS  Google Scholar 

  • Moore, G. E.: In vitro cultures of a pigmented hamster melanoma cell line. Exp. Cell Res.36, 422–423 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Morris, C. C.: Quantitative studies on the production of acid mucopolysaccharides by replicate cell cultures of rat fibroblasts. Ann. N. Y. Acad. Sci.86, 878–915 (1960).

    Article  PubMed  CAS  Google Scholar 

  • — Maintenance and loss in tissue culture of specific cell characteristics. Advanc. appl. Microbiol.4, 117–212 (1962).

    Article  CAS  Google Scholar 

  • Oettgen, H., Aoki, T., Old, L., Boyse, E. de Harven, E., Mills, G.: Suspension culture of a pigment-producing cell-line derived from a human malignant melanoma. J. nat. Cancer Inst.41, 827–843 (1968).

    PubMed  CAS  Google Scholar 

  • O’Malley, B.W., Kohler, P. O.: Hormonal induction of specific proteins in chick oviduct cell cultures. Biochem. biophys. Res. Commun.28, 1–7 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Oyama, V. I., Eagle, H.: Measurement of cell growth in tissue culture with a phenol reagent (Folin-Ciocalteau). Proc. Soc. exp. Biol. (N. Y.)91, 305–307 (1956).

    CAS  Google Scholar 

  • Paul, J.: The cancer cell in vitro. Cancer Res.22, 431–440 (1962).

    PubMed  CAS  Google Scholar 

  • Richardson, U. I., Tashjian, A. H., Jr., Levine, L.: Establishment of a clonal strain of hepatoma cells which secrete albumin. J. Cell Biol.40, 236–247 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Rohmsdahl, M. M.: Characteristics of cloned strains from human malignant melanoma cell lines grown in vitro. Fed. Proc.27, 720 (1968).

    Google Scholar 

  • Rose, G. G., Kumegawa, M., Nikai, H., Cattoni, M., Hu, F.: The HFH-18 mouse melanoma in roller tube, chamber, and circumfusion system cultures. Cancer Res.29, 2010–2033 (1969).

    PubMed  CAS  Google Scholar 

  • Sato, G. H., Yasumura, Y.: Retention of differentiated function in dispersed cell culture. Trans. N. Y. Acad. Sci.28, 1063–1079 (1966).

    PubMed  CAS  Google Scholar 

  • Schachtschabel, D.: Isolierung und Vermehrung von Melanin-bildenden Zellen des Harding-Passey-Melanoms in Zellkultur. Hoppe-Seylers Z. physiol. Chem.350, 1168 (1969a).

    CAS  Google Scholar 

  • — Biochemische und cytologische Untersuchungen zum Stoffwechsel, zur Teilung und Differenzierung von Zellen des Harding-Passey-Melanoms und Ehrlich-Ascites-Tumors in Zellkultur. In: Habilitationsschrift, p. 7–20. Marburg an der Lahn: Philipps-Universität 1969b.

    Google Scholar 

  • Schindler, R.: Die tierische Zelle in Zellkultur. In: P. Rentchnick (ed. in chief), Recent results in cancer research I, p. 26–33. Berlin-Heidelberg-New York: Springer 1965.

    Google Scholar 

  • — Day, M., Fischer, G. A.: Culture of neoplastic mast cells and their synthesis of 5-hydroxytryptamine and histamine in vitro. Cancer Res.19, 47–51 (1959).

    PubMed  CAS  Google Scholar 

  • Silagi, S.: Control of pigment production in mouse melanoma cells in vitro. Evocation and maintenance. J. Cell. Biol.43, 263–274 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Sloboda, A. E., Kopac, M. J.: A cytological analysis of the S 91 mouse melanoma in tissue culture. Ann. N. Y. Acad. Sci.100, 305–331 (1963).

    PubMed  CAS  Google Scholar 

  • Sugiura, K.: The effects of various physical and chemical factors on transplantable mouse melanoma. In: Biology of melanomas. N.Y.Acad. Sci. (Spec.Publ.)4, 369–387 (1948).

    CAS  Google Scholar 

  • Whittaker, J. R.: Changes in melanogenesis during the dedifferentiation of chick retinal pigment cells in cell culture. Develop. Biol.8, 99–127 (1963).

    Article  CAS  Google Scholar 

  • — Loss of melanotic phenotype in vitro by differentiated retinal pigment cells: demonstration of mechanisms involved. Develop. Biol.15, 553–574 (1967).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institut für Physiologische Chemie der Philipps-Universität Marburg a. d. Lahn, Deutschland

    D. Schachtschabel

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Mit Untersützung der Deutschen Forschungsgemeinschaft (Scha 176/1 und 176/2).

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Schachtschabel, D. Spezifische Zellfunktionen von Zell- und Gewebekulturen. Virchows Arch. Abt. B Zellpath. 7, 27–36 (1971). https://doi.org/10.1007/BF02892076

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  • DOI: https://doi.org/10.1007/BF02892076

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