Souhrn
Buňky kvasinek s volutinovými granuly vykazujícími metachromatickou reakci obsahovaly kromě fosforu vázaného v buněčných bílkovinách a kromě stabilně přítomných elektroforeticky pohyblivých sloučenin další specifickou sloučeninu fosforu o vysoké elektroforetické pohyblivosti. Tato nebílkovinná sloučenina, prokazatelně nepřítomná v buňkách bez volutinu, byla oddělena pomocí elektroforesy na papíře a stanovena jako metafosfát. Toto zjištění nevylučuje ovšem moznost účasti ribonukleové kyseliny při tvorbě volutinových granulí.
Резюме
Дрожжевые клетки с во лютином, которые дава ли метахроматичеокую р еакцию, содержали, кроме фосфора клеточ ных белков и кроме пос тоянно присутствующих в них электрофоретиЧески подвижных соединени й, другое соединение ф осфора с высокой электрофоре тической подвижностью. Это не б елковое соединение, к оторое не содержится в клетках без волютина, было определено как м етафосфат. Это открыт ие не исключает возможнос ти участия в синтезе волютина и рибонукле иновой кислоты.
Summary
Yeast cells with volutin showing a metachromatic reaction contained, in addition to the phosphorus bound in cell proteins and to its electrophoretically mobile compounds which are constantly present, another specific phosphorus compound of high electrophoretic mobility. This non-protein compound — evidently absent in volutin-free cells—was identified as metaphosphate and separated by means of paper electrophoresis. This finding does not exclude the possibility that ribonucleic acid participates in the formation of volutin granules.
This is a preview of subscription content, log in via an institution to check access.
Literatura
Bel’ozerskij, A. N.:Chimičeskoje svojstvo volutina. Mikrobiologija 14: 29, 1945.
Brandt, K.:Physiologische Chemie und Cytologie der Presshefe. Protoplasma 36: 77, 1941.
Caspersson, T., Brandt, K.:Nucleotidumsatz und Wachstum bei Presshefe. Protoplasma 35: 517, 1941.
Damle, S. P., Krishnan, P. S.:Studies on the role of metaphosphate in molds. I. Quantitative studies on the metachromatic effect of metaphosphate. Arch. Bioch. Biophys. 49: 58, 1954.
Duguid, J. P., Smith, J. W., Wilkinson, J. F.:Volutin production in Bacterium aerogenes due to development of an acid reaction. J. Pathol. Bacteriol. 67: 289, 1954.
Ebel, J. P.:Sur le dosage des métaphosphates dans les microorganismes par hydrolyse différentielle; technique et application aux levures. C. R. Ac. Sci. 226: 2184, 1948.
Ebel, J. P.:Participation de l’acide métaphosphorique à la constitution des bactéries et des tissus animaux. C.R. 228: 1312, 1949.
Ebel, J. P., Volmar Y.:Chromatographie sur papier des ortho, pyro, méta et polyphosphates. C. R. Ac. Sci. 233: 415, 1951.
Jeener, R., Brachet, J.:Synthesis of nucleic acid by yeast. Relations with fermentation and respiration. Bull, classe sci. Acad. Roy. Belg. 29: 476, 1943.
Juni, E., Kamen, M. D., Reiner, J. M., Spiegelman, S.:Turnover and distribution of phosphate compounds in yeast metabolism. Arch. Bioch. 18: 387, 1948.
Kornberg, S. R., Kornberg, A.:Anorganictripolyphosphate and trimetaphosphate in yeast extracts. Feder. Proc. 13: 244, 1954.
Mackie, T. J., Me Cartney, J. E.:Handbook of practical bacteriology. Edinburgh 1950.
Malmgren, H.:Enzymatic breakdown of poly metaphosphate. Särtn. Svensk Kern. Tidskr. 64: 27, 1952.
Mann, T.:Studies on the metabolism of mould fungi. 1. Phosphorus metabolism in moulds. Biochem. J. 38: 339, 1944a.
Mann, T.:Studies on the metabolism of mould fungi. 2. Isolation of pyrophosphate and metaphosphate from Aspergillus niger. Biochem. J. 38: 345, 1944b.
Meyer, A.:Orientierende Untersuchung über Verbreitung, Morphologie und Chemie des Volutins. Bot. Zeit. 62: 113, 1904.
Schmidt, G., Hecht, L., Thannhauser, S. J.:The enzymatic formation and the accumulation of large amounts of a metaphosphate in bakesr’ yeast under certain conditions. J. Biol. Chem. 166: 775, 1946.
Schmidt, G., Hecht, L., Thannhauser, S. J.:The effect of potassium ions on the absorption of orthophosphate and the formation of metaphosphate by bakers’ yeast. J. Biol. Chem. 178: 733, 1949.
Schumacher, J.:Welche chemische Substanz baut die Polkörnchen des Diphtherie Bazillus auf? Zbl. Bakter. I. Abt. 88: 362, 1922.
Schumacher, J.:Über den Nachweis des Bakterienkerns und seine chemische Zusammensetzung. Zbl. Bakter. I. Abt. 97: 81, 1926.
Smith, J. W., Wilkinson, J. F., Duguid, J. P.:Volutin production in Aerobacter aerogenes due to nutrient imbalance. J. Bact. 68: 450, 1954.
Sommer, A. L., Booth, T.:Meta and pyrophosphate within the algal cells. Plant Physiol. 13: 199, 1938.
Van Herwenden, M.:On the nature and significance of volutin in yeast cells. Ak. Amst.,Proc, 20: 70, 1918.
Wiame, J. M.:Remarque sur la métachromasie des cellules de levure. C. R. Soc. Biol. 140: 895, 1946a.
Wiame, J. M.:Basophilie et métabolisme du phosphore chez la levure. Bull. Soc. Chim. Biol. 28: 552, 1946b.
Wiame, J. M.:Y east metaphosphate. Feder. Proc. 6: 302, 1947a.
Wiame, J. M.:The metachromatic reaction of hexametaphosphate. J. Am. Chem. Soc. 69: 3146, 1947b.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stárka, J., Závada, J. Vztah metafosfátu k tvorbě volutinu u kvasinek. Ceskoslovenská Mikrobiologie 1, 70–73 (1956). https://doi.org/10.1007/BF02891827
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02891827