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Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology

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Chinese Science Bulletin

Abstract

Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNG-induced nontargeted mutation frequency enhanced significantly compared with control (P< 0.05). The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.

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Authors and Affiliations

  1. Department of Pathophysiology, Zhejiang Medical University, 310031, Hangzhou, China

    Wenwei Hu, Yingnian Yu, Xingruo Chen, Tao Song & Haiyang Xie

Authors
  1. Wenwei Hu
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  2. Yingnian Yu
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  3. Xingruo Chen
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  4. Tao Song
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  5. Haiyang Xie
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Hu, W., Yu, Y., Chen, X. et al. Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology. Chin.Sci.Bull. 44, 533–537 (1999). https://doi.org/10.1007/BF02885542

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  • DOI: https://doi.org/10.1007/BF02885542

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