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Application of radiation hybrid in gene mapping

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Abstract

Radiation hybrid (RH) mapping technique was exploited to determine chromosome locations of 26 human novel full length cDNAs recently cloned. All these cDNA clones were isolated from human cord blood CD 34+ cells and may be related to regulation of hematopoiesis. 23 genes were successfully mapped to chromosomal positions, while RH analyses were not possible in the remaining 3 cases. RH technique is indeed a powerful tool for mapping novel cDNA sequences due to its rapidity, precision, convenience and reproducibility.

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Authors and Affiliations

  1. Key Laboratory for Human Genome Research and Shanghai Institute of Hematology, Rui-Jin Hospital, Shanghai Second Medical University, 200025, Shanghai, China

    Kaili He, Baiwei Gu, Qinghua Zhang, Gang Fu, Jisheng Wu, Zeguang Han, Wenjun Cao, Jun Zou, Mao Mao, Jianxiang Liu, Zhu Chen & Saijuan Chen

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  1. Kaili He
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  2. Baiwei Gu
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  3. Qinghua Zhang
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  4. Gang Fu
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  5. Jisheng Wu
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  6. Zeguang Han
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  7. Wenjun Cao
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  8. Jun Zou
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  9. Mao Mao
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  10. Jianxiang Liu
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  11. Zhu Chen
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  12. Saijuan Chen
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He, K., Gu, B., Zhang, Q. et al. Application of radiation hybrid in gene mapping. Sci. China Ser. C.-Life Sci. 41, 644–649 (1998). https://doi.org/10.1007/BF02882907

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  • DOI: https://doi.org/10.1007/BF02882907

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