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Long-term preservation of yeast cultures in liquid nitrogen

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Abstract

Nineteen strains of taxonomieally diverse yeast species tested survived freezing and subsequent five-year storage in liquid nitrogen at ™196 °C, using a medium M 2 composed of malt extract, yeast extract, peptone, calf serum and dimethyl sulfoxide. Viability of the yeast cultures after long-term storage ranged from 5 to 97 % (average 62 %) compared with the viability of the cultures prior to freezing. The use of liquid nitrogen refrigeration for preserving yeast cultures is strongly advocated.

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Authors and Affiliations

  1. Institute of Parasitology, Czechoslovak Academy of Sciences, 166 32, Prague

    Z. Hubálek

  2. Institute of Chemistry, Slovak Academy of Sciences, 809 33, Bratislava

    A. Kocková-Kratochvílová

Authors
  1. Z. Hubálek
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  2. A. Kocková-Kratochvílová
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Hubálek, Z., Kocková-Kratochvílová, A. Long-term preservation of yeast cultures in liquid nitrogen. Folia Microbiol 27, 242–244 (1982). https://doi.org/10.1007/BF02877123

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  • DOI: https://doi.org/10.1007/BF02877123

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