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Determination of antibodies to diphtheria and tetanus toxoid by latex agglutination technique

Определение противодифтерийных и противостолбнячных антител техникой латексовой агглютинации

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Abstract

A micromethod of latex agglutination was worked out for determination of antibodies to diphtheria and tetanus toxoids. It is suitable for pediatrics since a minimum amount of serum (0.05 ml.) is required, which can be used without being inactivated or absorbed. Particles of polystyrene latex of Czechoslovak production were used as antigen carriers thus enabling better standardization of the technique. The particles are well defined both in physical and chemical terms and replace red blood cells in passive hemagglutination. The method was compared with passive hemagglutination in a dynamic study of children inoculated at monthly intervals with the trivalent vaccine Alditepera.

Abstract

Был разработан микрометод агглютинации с использованием латекса для определения антител против дифтерии и столбняка, удобный для педиатрических отделений ввиду минимального потредления сыворотки (только 0,05 мл), которую нет необходимости ни инактивировать, ни истощать. В качестве носитель антигена при этом применяются частицы полистиренового латекса чехословацкого производства, что позволяет лучше стандартизировать метод. Эти физически и химически определенные частицы заменяют в данном случае эритроциты, применяемые для пассивной гемагглютинации. Метод был проверен путем сравнения с пассивной гемагглютинацией и динамического прослеживания группы детей, которые с 1-месячными интервалами инокулировались трехвалентной вакциной по Alditepera.

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Authors and Affiliations

  1. Institute for the Care of Mother and Child, Prague 4

    V. Jouja

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  1. V. Jouja
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Jouja, V. Determination of antibodies to diphtheria and tetanus toxoid by latex agglutination technique. Folia Microbiol 10, 341–345 (1965). https://doi.org/10.1007/BF02875702

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  • DOI: https://doi.org/10.1007/BF02875702

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