Abstract
Fractions of the cell wall ofSaccharomyces cerevisiae where a α-chtin-glucan composition was established, were examined under the electron microscope as well as with phase fluorescence. The results indicate that the α-chitin-glucan complex shows a fibrillar arrangement and is localized in the socalled encircling region of the bud scar which has a tear-like appearance and is electron-transparent in ultrathin sections. There are indications of the presence of chitin even in the primary septum.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agar H. D., Douglas H. C.: Studies of budding and cell wall structure of yeast. Electron microscopy of thin sections.J. Bacteriol.70, 427 (1955).
Bacon J. S. D., Davidson E. D., Taylor I. F.: The location of chitin in the yeast cell wall.Biochem. J.101, 36C (1966).
Bartholomew J. W., Mittwer T.: Demonstration of yeast bud scars with the electron microscope.J. Bacteriol.65, 272 (1953).
Barton A. A.: Some aspects of cell division inSaccharomyces cerevisiae.J. Gen. Microbiol.4,84 (1950).
Beran K.: Budding of yeast cells, their scars and ageing.Advan. Microbiol. Physiol.2, 143 (1968).
Beran K.: Infrared spectral analysis of the cell wall. The application of the method in the study of chitin in the cell walls of the yeastSaccharomyces cerevisiae.Acta Facultatis Medicae Universitatis Brunensis37, 171 (1970). Proceedings of the IInd International Symposium on Yeast protoplast. Brno 1968.
Beran K., Holan Z., Baldrián J.: Chitin-glucan complex in yeastSaccharomyces cerevisiae. I. IR and X-ray analysis.Folia Microbiol.17, 322 (1972).
Cabib E., Bowers B.: Chitin and yeast budding. Localization of chitin in yeast bud scars.J. Biol. Chem.246, 152 (1971).
Cabib E., Farkaš V.: The control of morphogenesis: An enzymatic mechanism for the initiation of septum formation in yeast.Proc. Natl. Acad. Sci. USA68, 2052 (1971).
Frey-Wyssling A.: Anisotropic diffusion of direct dyes in fibres.J. Polymer. Sci.2, 314 (1947).
Frey-Wyssling A.: Submicroscopic morphology of protoplasm. Elsevier Publ. Comp., Amsterdam 1953.
Frey-Wyssling A.: Die pflanzliche Zellwand. Springer Verlag, Berlin 1959.
Houwink A. L., Kreger D. R.: Observation of the cell wall of yeast. An electron microscope and X-ray diffraction study.Anton Leeuwenhoek J. Microbiol.19, 1 (1953).
Kreger van Rij N. J. W., Veenhuis M.: A study of vegetative reproduction inEndomycopsis platypodis by electron microscopy.J. Gen. Microbiol.58, 341 (1969).
Kreger van Rij N. J. W., Veenhuis M.: A comparative study of the cell wall structure ofBasidomycetous and related yeasts.J. Gen. Microbiol.68, 87 (1971).
Maeda H., Ishida N.: Specificity of binding of hexapyranosyl polysaccharides with fluorescent brightener.J. Biochem.62, 276 (1967).
Marchant R., Smith D. G.: Bud formation inSaccharomyces cerevisiae and comparison with the mechanism of cell division in other yeast.J. Gen. Microbiol.53, 163 (1968).
Marchessault R. H., Sarko A.: X-ray structure of polysaccharides.Adv. Carbohydr. Chem.22, 421 (1967).
Mundkur B.: Electron microscopical studies of freeze-dried yeast. I. Localization of polysaccharides.Exp. Cell Res.20, 28 (1960).
Reynolds E. S.: The use of lead citrate at high pH as an electronoptique strain in electron microscopy.J. Cell Biol.17, 208 (1963).
Roelofsen P. A.: The Plant Cell Wall. Borntreager, Berlin 1959.
Rudall K. M.: Chitin and its association with other molecules.J. Polymer. Sci. Part C,28, 83 (1969).
Sarko A., Marchessault R. H.: Supermolecular structure of polysaccharides.J. Polymer Sci. Part C28, 317 (1969).
Seichertová O., Beran K., Ludvík J.: Contribution to the structure of the cell wall in the area of the bud scar inSaccharomyces cerevisiae.Anton Leeuwenhoek J. Microbiol.35, B 13 (1969). Proceeding of the IIIrd International symposium on Yeast. Delft-Hague 1969.
Sentandreu R., Northcote D. H.: The formation of buds in yeast.J. Gen. Microbiol.55, 393 (1969).
Shannon J. L., Rothman A. H.: Transverse septum formation in budding cells of the yeast-like fungusCandida albicans.J. Bacteriol.106, 1026 (1971).
Sterling C.: Starch-primulin fluorescence.Protoplasma59, 180 (1964).
Streiblová E.: PhD thesis, Institute Microbiology, Prague 1966.
Streiblová E., Beran K.: Demonstration of yeast scars by fluorescence microscopy.Exp. Cell Res.30, 603 (1963).
Streiblová E., Beran K.: On the question of vegetative reproduction in apiculate yeast.Folia Microbiol.10, 352 (1965).
Vitols E., North R. J., Linnane A. V.: Studies on the oxidative metabolism ofSaccharomyces cerevisiae. I. Observations on the fine structure of the yeast cell.J. Biophys. Biochem. Cytol.9, 689 (1961).
Author information
Authors and Affiliations
Additional information
The results described here were presented at the Third International Symposium on Yeasts, Delft, 1969. The manuscript was prepared on the basis of photographs and observations left by the deceased first author.
Rights and permissions
About this article
Cite this article
Seichertová, O., Beran, K., Holan, Z. et al. The chitin-glucan complex ofSaccharomyces cerevisiae . Folia Microbiol 18, 207–211 (1973). https://doi.org/10.1007/BF02872858
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02872858