Abstract
Two interfertile species ofCucurbita, the wildC. texana and the domesticatedC. pepo (var.medullosa), were hybridized to elucidate the genetic basis of inter-specific differences in isozyme phenotypes. Presented here are descriptions of phenotypes associated with 6 enzyme systems (GOT, IDH, MDH, PER, PGI, PGM). Genetic interpretation for 6 gene loci and 12 alleles is based on F2 segregation data while 10 additional loci and 15 alleles are presumed on the basis of phenotypic variation found throughout theC. pepo complex. A coefficient of genetic identity calculated for single populations of each taxon was within the range that traditionally separates species. However, observations on phenotypes in both taxa leave the taxonomic delimitation of C. texana unresolved. The complexity of banding patterns in some enzyme systems suggests a polyploid origin for the genus Cucurbita.
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Literature Cited
Bailey, L. H. 1943. Species ofCucurbita. Gentes Herb. 6: 267–322.
Berg, B. M. van den, and H. J. W. Wijsman. 1981. Genetics of the peroxidase isoenzymes inPetunia. Part 1: Organ specificity and general genetic aspects of the peroxidase isoenzymes. Theor. Appl. Genet. 60:71–76.
Cardy, B. J., C. W. Stuber, and M. M. Goodman. 1980. Techniques for starch gel electrophoresis of enzymes from maize (Zea mays L.). Inst. Statistics Mimeo No. 1317, North Carolina State Univ., Raleigh, NC.
Conard, N., D. L. Asch, N. B. Asch, D. Elmore, H. Grove, M. Rubin, J. A. Brown, M. D. Wiant, K. B. Farnsworth, and T. G. Cook. 1984. Accelerator radiocarbon dating of evidence for prehistoric horticulture in Illinois. Nature 308: 443–446.
Correll, D. S., and M. C. Johnston. 1979. Manual of the Vascular Plants of Texas. Univ. Texas, Dallas, TX.
Crawford, D. J. 1983. Phylogenetic and systematic inferences from electrophoretic studies.In S. D. Tanksley and T. J. Orton, ed, Isozymes in Plant Genetics and Breeding, Part A, p. 257–287. Elsevier, Amsterdam.
Dane, F. 1983. Cucurbits.In S. D. Tanksley and T. J. Orton, ed, Isozymes in Plant Genetics and Breeding, Part B, p. 369–390. Elsevier, Amsterdam.
Decker, D. S. 1985. Numerical analysis of allozyme variation inCucurbita pepo. Econ. Bot. 39: 300–309.
Denna, D. W., and M. B. Alexander. 1975. The isoperoxidases ofCucurbita pepo L.In C. L. Markert, ed, Isozymes II: Physiological function, p. 851–864. Academic Press, New York.
Erwin, A. T. 1938. An interesting Texas cucurbit. Iowa State Coll. J. Sci. 12: 253–255.
Garcia, P., M. Perez de la Vega, and C. Benito. 1982. The inheritance of rye seed peroxidases. Theor. Appl. Genet. 61: 341–351.
Goodman, M. M., and C. W. Stuber. 1983. Isozymes of maize.In S. D. Tanksley and T. J. Orton, ed, Isozymes in Plant Genetics and Breeding, Part B, p. 1–33. Elsevier, Amsterdam.
Gottlieb, L. D. 1977. Electrophoretic evidence and plant systematics. Ann. Missouri Bot. Gard. 64: 161–180.
—. 1981. Electrophoretic evidence and plant populations. Prog. Phytochem. 7:1–46.
—. 1982. Conservation and duplication of isozymes in plants. Science 216: 373–380.
—, and N. F. Weeden. 1981. Correlation between subcellular location and phosphoglucose isomerase variability. Evolution 35: 1019–1022.
Gray, A. 1868. Field, Forest and Garden Botany. American Book, New York.
Heiser, C. B., {jrJr.} 1979. The Gourd Book. Univ. Oklahoma Press, Norman, OK.
Kay, M., F. B. King, and C. K. Robinson. 1980. Cucurbits from Phillips Spring: new evidence and interpretations. Amer. Antiquity 45: 806–822.
Nei, M. 1972. Genetic distance between populations. Amer. Naturalist 106: 283–292.
Rick, C. M., S. D. Tanksley, and J. F. Fobes. 1979. A pseudoduplication inLycopersicon pimpinellifolium. Proc. Natl. Acad. Sci. USA. 76: 3435–3439.
Robinson, R. W., H. M. Munger, T. W. Whitaker, and G. W. Bohn. 1976. Genes of the Cucurbitaceae. HortScience 11: 554–568.
Singh, A. K. 1979. Cucurbitaceae and polyploidy. Cytologia 44: 897–905.
Smith, E. B. 1978. An Atlas and Annotated Checklist of the Vascular Plants of Arkansas. Univ. Arkansas, Fayetteville, AR.
Suiter, K. A., J. F. Wendel, and J. S. Case. 1983. Linkage-1: a Pascal computer program for the detection and analysis of genetic linkage. J. Heredity 74: 203–204.
Whitaker, T. W., and W. P. Bemis. 1975. Origin and evolution of the cultivatedCucurbita. Bull. Torrey Bot. Club 102: 362–368.
Wilson, H. D. 1981. Genetic variation among South American populations of tetraploidChenopodium sect. Chenopodium subsect. Cellulata. Syst. Bot. 6: 380–398.
—, S. C. Barber, and T. Walters. 1983. Loss of duplicate gene expression in tetraploidChe-nopodium. Biochem. Syst. Ecol. 11: 7–13.
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Kirkpatrick, K.J., Decker, D.S. & Wilson, H.D. Allozyme differentiation in theCucurbita pepo complex:C. pepo var.medullosa vs.C. texana . Econ Bot 39, 289–299 (1985). https://doi.org/10.1007/BF02858798
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DOI: https://doi.org/10.1007/BF02858798