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An effective method for culturing pollen tubes of potato

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Abstract

In vitro culture of pollen tubes is a convenient way to distinguish inviable pollen from stylar incompatibilities which prevent pollen tube growthin vivo. This usefulness ofin vitro pollen tube culture depends on how successful the technique is in promoting optimal pollen tube growth. Experiments were conducted to compare the effects of the type of sugar and method of aeration of cultures ofSolanum species (potato) pollen. Lactose was superior to sucrose in promoting pollen germination and pollen tube growth on hanging drop cultures. Germination and growth were greatly enhanced when pollen was cultured in actively aerated lactose medium. Since pollen growth comparable to that occurringin vivo was observed using lactose medium with active aeration, this technique may be useful in various types of research related to reproductive biology.

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Authors and Affiliations

  1. USDA, Agricultural Research Service, USA

    J. B. Bamberg (Geneticist) & R. E. Hanneman (Research Geneticist)

  2. Inter-Regional Potato Introduction Station, 54235, Sturgeon Bay, WI, USA

    J. B. Bamberg (Geneticist)

  3. Department of Horticulture, University of Wisconsin, 53706, Madison, WI, USA

    R. E. Hanneman (Research Geneticist)

Authors
  1. J. B. Bamberg
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  2. R. E. Hanneman
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Additional information

Cooperative investigation of the U.S. Department of Agriculture, Agricultural Research Service and the Wisconsin Experiment Station. Supported in part by the USDA/Cooperative States Research Service Competitive Grant No. 83-CRCR-1-1253. The use of trade or brand names in this research does not constitute an endorsement by the USDA or University of Wisconsin.

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Bamberg, J.B., Hanneman, R.E. An effective method for culturing pollen tubes of potato. American Potato Journal 68, 373–379 (1991). https://doi.org/10.1007/BF02853618

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  • DOI: https://doi.org/10.1007/BF02853618

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