Abstract
Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae, and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of the starfish had already regionalized before the GV break-down.
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References
Bates, W. R. and W. R. Jeffery, 1987. Localization of axial determinants in the vegetal pole region of ascidian eggs.Dev. Biol. 124: 65–76.
Boveri, T., 1901. Die Poloritat von Ovozyte, Ei und Larve des Strongylocentrotus lividus.Zool. Jahrb. Abt. Anat. Ontog. Tiere 14: 630–653.
Capco, D. G. and M. D. Mecca, 1988. Analysis of proteins in the peripheral and central regions of amphibian oocytes and eggs.Cell Differ.23: 155–164.
Chabry, L. 1887 Contribution a l'embryologie normale et teratologique des Ascidies simples.J. Anat. Physiol. 23: 167–319.
Conklin, E. G., 1905. The organization and cell lineage of the asicidian egg.J. Acad. Natl. Sci. Philadelphia 13: 1–119.
Costello, D. P., 1940. The fertilizability of nucleated and non-nucleated fragments of centrifuged Nereis eggs.J. Morph. 66: 99–144.
Crowther, R. J. and J. R. Whittaker, 1986. Developmental autonomy of presumptive notochord cells in partial embryos of an ascidian.Int. J. Invertebr. Reprod. Dev. 9: 253–261.
Davidson, E. H., 1976. Gene Activity in Early Development. Academic Press, New York. pp.245–318.
Davidson, E. H., 1986. Gene Activity in Early Development (third edition). Academic Press. Inc. pp. 409–524.
Delage, Y., 1901. Etudes experimentales sur la maturation cytoplasimque et sur la parthenogenese articielle chez les echinodermes.Arch. Zool. Exp. 3ser 9: 285–326.
Eddy, L. M., 1975. Germ plasm and the differentiation for the germ line.Int. Rev. Cytol. 43: 229–280.
Harnly, M. H., 1926. Localization of the micromere materials in the cytoplasm of the egg of Arbacia.J. Exp. Zool. 45: 319–333.
Hirai, S., Kubota, J. and Kanatani, H., 1971. Induction of cytoplasmic maturation by 1-methyladenine in starfish oocytes after removal of the germinal vesicle.Exp. Cell Res. 68: 137–143.
Hirai, S., Nagahama. Y., Kishimoto, T. and Kanatani, H., 1981. Cytoplasmic maturity revealed by the structural changes in incorported spermatizoon during the course of starfish oocyte maturation. Develop. Growth and Differ.23: 465–478.
Horstadius. S., 1928. Uber die Determination des Keines bei Echinideremen.Acta. Zool. 9: 1–191.
Horstadius. S., 1937. Investigations as to the localization of the micromere, the skeleton and the entodermforming material in the unfertilized egg of Arbacia punctulata.Biol. Bull. 73: 295–316.
Horstadius. S., 1939. The mechanics of sea urchin development, studied by operative methods.Biol. Rev. 14: 132–179.
Iwamatsu. T., 1966. Role of germinal vesicle materials on the acquisition of developmental capacity of the fish oocyte.Embryologia 9: 205–221.
Jeffery, W. R. and W. R. Bates. 1987. Ooplasmic segregation in the ascidianStyela.In “Molecular Biology of Fertilization” (H. Schatten and G. Schatten. Eds.) Academic Press, New York.
Jeffery, W. R., 1984. Pattern formation by ooplasmic segregation in ascidian eggs.Biol. Bull. 16: 277–289.
Kanatani, H., 1973. Maturation-inducing substance in starfishes.Int. Rev. Cytol. 35: 253–298.
Katagiri, C. and M. Moriya, 1976. Spermatozoan response to the toad egg matured after removal of germinal vesicle.Dev. Biol. 50: 235–241.
Klag, J. J. and G. A. Ubbels, 1975. Regional morphological differentiation in the fertilized egg ofDiscoglossus pictus (anura).Differentiation 3: 15–20.
Mahowald, A. P., C. D. Allis, K. M. Karrer, E. M. Underwood and G. L. Waring, 1979. Germ plasm and pole cells ofDrosophila.In “Determinants of Spatial Organization” (S. Subtelny and I. R. Konigsbery, Eds.). Academic Press. New York, pp. 127–146.
Maruyama, Y. K., Y. Kakaseko and S. Yagi, 1985. Localization of cytoplasmic determinants responsible for primary mesenchyme formation and gastrulation in the unfertilized egg of the sea urchin Hemicentrotus pulcherrimus.J. Exp. Zool. 236: 155–163.
Michael, P., 1984. Are the primordial germ cells (PGC) in Urodeles formed by the inductive action of the vegetative yolk mass?Dev. Biol. 103: 109–166.
Morgan, T. H., 1934. Embryology and Genetics (Columbia University Press, New York). 100 pp.
Niki, Y., 1984. Developmental analysis of the grandchildless (gs(1) N26) mutation inDrosophila melanogaster. Abnormal cleavage patterns and defects in pole cell formation.Dev. Biol. 103: 182–189.
Nishikata, T., I. Mita Miyazawa, T. Deno and N. Satoh, 1987. Muscle cell differentiation in ascidian embryos analyzed with a tissue-specific monoclonal antibody.Development 99: 163–171.
Tung, T. C., Ku, S. H. and Y. F. Y. Tung, 1941. The development of the ascidian egg centrifuged before fertilization.Biol. 2: 153–168.
Tung, T. C., S. C. Wu and Y. F. Yen, 1981. Regionalization of the egg cytoplasm of the lower chordates before fertilization.American Zoologist 24: 419.
Uzman, J. A. and W. R. Jeffery, 1986. Cytoplasmic determinants for cell lineage specification in ascidian embryos.Cell Differ.18: 215–224.
Whittaker, R. J., 1973. Segregation during ascidian embryogenesis of egg cytoplasmic information for tissue specific enzyme development. Proc. Natl. Acad. Sci. U.S.A.70: 2096–2100.
Whittaker, J. R., 1976. Cytoplasmic determinants of tissue differentiation in the ascidian egg.In “Determinants of Spatial Organization” (S. Subtelny and Konigsberg, eds.) Academic Press, New York, pp. 29–51.
Wilson, E. B., 1903. Experiments on cleavage and localization in the nemertine egg. Roux' Arch. Entwicklungsmech.16: 411–461.
Wilson, E. B., 1925. The Cell in Development and Heredity, (MacMillan, London). pp. 1035–1121.
Wu S. C., 1986. Localization of adult tropomyosin in the developing oocytes of amphioxus. Branchiostoma belcheri. Int. Minisymp. On Develop. Biol. Proc., Beijing, 114–115.
Wylie, C. C., D. Brown, S. F. Godsave, J. Quarby, and J. Heasman, 1985. The cytoskeleton ofXenopus oocytes and its role in development.J. Embryol. Exp. Morph. 89 supplement, 1–15.
Yamada, H. and S. Hirai, 1984. Role of contents of the germinal vesicle in male pronuclear development and cleavage of starfish oocytes.Develop. Growth and Differ.26: 479–487.
Yatsu, N., 1904. Aster formation in enucleated egg-fragments of Cerebratus.Science. N. S. 11: 889–890.
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Contribution No. 1722 from the Institute of Oceanology, Academia Sinica
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Shicui, Z., Xianhan, W., Jing, Z. et al. Cytoplasmic regionalization in starfish oocyte occurrence and localization of cytoplasmic determinants responsible for the formation of archenteron and primary mesenchyme in starfish (asterias amurensis) oocytes. Chin. J. Ocean. Limnol. 8, 263–272 (1990). https://doi.org/10.1007/BF02849666
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DOI: https://doi.org/10.1007/BF02849666