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Production of glutamic acid byArthrobacter globiformis: Influence of cultural conditions

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Abstract

Arthrobacter globiformis isolated from Burdwan soil excretes glutamic acid in a glucose mineral salt medium with suboptimal level of biotin. Glutamate begins to accumulate in the medium from the mid-exponential phase of growth and its excretion could be prolonged by adjustment of pH to the neutral range. Among the different carbon and nitrogen sources tested glucose (8%) and ammonium nitrate (0.53%), respectively, were found to be most suitable. Molasses could not be used as a substitute for glucose even if antibiotics or Tween 80 are incorporated in the medium. Bacitracin (1 μg/mL) stimulated glutamate excretion. Atemperature of 28°C and an inoculum dose of 4% were optimal for production. Under optimal conditions, in the flask culture the isolate excreted 16.1 g glutamic acid per litre in 120 h. Glutamic acid isolated from the fermented broth was found to be purel-diastereomer.

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  1. D. K. Roy

    Present address: Central Tasar Research & Training Institute, BSM & TC, Ambikapur, Dt. Surguja, M.P., India

Authors and Affiliations

  1. Department of Botany, Burdwan University, 713 104, Burdwan, India

    D. K. Roy & S. P. Chatterjee

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  1. D. K. Roy
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  2. S. P. Chatterjee
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Roy, D.K., Chatterjee, S.P. Production of glutamic acid byArthrobacter globiformis: Influence of cultural conditions. Folia Microbiol 34, 11–24 (1989). https://doi.org/10.1007/BF02821319

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  • DOI: https://doi.org/10.1007/BF02821319

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