Skip to main content
Log in

Salmonellosis: Lessons drawn from a germ-free pig model

  • Immunology
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The germ-free pig model is shown to be useful for studying salmonellosis. The immune status of germ-free and infected gnotobiotic piglets is described. The regulatory role of cytokine is discused and compared with our experimental findings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Abraham S.N., Malaviya R.: Mast cells in infection and immunity.Infect. Immun. 65, 3501–3508 (1997).

    PubMed  CAS  Google Scholar 

  • Conlan J.W.: Critical roles of neutrophils in host defense against experimental systemic infections of mice byListeria monocytogenes, Salmonella typhimurium, andYersinia enterocolitica.Infect. Immun. 65, 630–635 (1997).

    PubMed  CAS  Google Scholar 

  • Cukrowska B.: B cell development during early ontogeny: study on pig fetuses, germ-free piglets and preterm human infants. PhD Thesis. Institute of Microbiology, Prague 1996.

    Google Scholar 

  • Cukrowska B., Šinkora J., Řeháková Z., Šinkora M., Šplíchal I., Tučková L., Avrameas S., Saalmüller A., Barot-Ciorbaru R., Tlaskalová-Hogenová H.: Isotype and antibody specificity of spontaneously formed immunoglobulins in pig fetuses and germ-free piglets: production by CD5 B cells.Immunology 88, 611–617 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Dignass A.U., Stow J.L., Babyatsky M.W.: Acute epithelial injury in the rat small intestinein vivo is associated with expanded expression of transforming growth factor α and β.Gut 38, 687–693 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Dlabač V., Talafantová M., Mandel L., Štěpánková R.: Factors influencing the fate ofEscherichia coli andSalmonella typhimurium in germfree piglets and rats.7th Internat. Congr. Mucosal Immunology (Prague), Abstracts p. 55 (1992).

  • Dlabač V., Trebichavský I., Řeháková Z., Hofmanová B., Šplíchal I., Cukrowska B.: Pathogenicity and protective effect of rough mutants ofSalmonella species in germ-free piglets.Infect. Immun. 65, 5238–5243 (1997).

    PubMed  Google Scholar 

  • Ferenčík M., Bergendi L., Mandel L., Kovářů F., Štefanovič J.: Lysosomal enzyme activities in polymorphonuclear leukocytes, macrophages, serum and spleen of conventional, germ-free and antigen-free Minnesota miniature swine.Folia Microbiol. 30, 65–75 (1985).

    Article  Google Scholar 

  • Fresno M., Kopf M., Rivas L.: Cytokines and infectious diseases.Immunol. Today 18, 56–58 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Gruenheid S., Pinner E., Desjardins M., Gros P.: Natural resistance to infection with intracellular pathogens: the Nramp 1 protein is recrutied to the membrane of the phagosome.J. Exp. Med. 185, 717–730 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Herfarth H.H., Mohanty S.P., Rath H.C., Tonkonogy S., Sartor R.B.: Interleukin-10 suppresses experimental chronic, granulomatous inflammation induced by bacterial cell wall polymers.Gut 39, 836–845 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Hornaeche C.E., Harrington K.A., Joysey H.S.: Natural resistance to Salmonellae in mice: control by genes within the major histocompatibility complex.J. Infect. Dis. 152, 1050–1056 (1985).

    Google Scholar 

  • Letterio J.J., Roberts A.B.: Molecule of the month TGF-β: a critical modulator of immune cell function.Clin. Immunol. Immunopathol. 84, 244–250 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Mandel L., Trebichavský I., Šplíchal I., Schulze J.: Stimulation of intestinal immune cells byE. coli in gnotobiotic piglets, pp. 463–464 inAdvances in Mucosal Immunology (J. Mestecky, Ed.). Plenum Press, New York 1995.

    Google Scholar 

  • Mandel L.: Rearing of germ-free pigs, pp. 1544–1546 inImmunology Methods Manual (I. Lefkovits, Ed.). Academic Press, London 1997.

    Google Scholar 

  • Marinaro M., Boyaka P.N., Finkelman F.D., Kiyono H., Jackson R.J., Jirillo E., McGhee J.R.: Oral but not parenteral interleukin (IL)-12 redirects T helper 2 (Th2)-type responses to an oral vaccine without altering mucosal IgA responses.J. Exp. Med. 185, 415–427 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Mouton D., Biozzi G., Bouchillier Y., Stiffel C.: Phagocytosis of Salmonellae by reticuloendothelial cells of new-born piglets lacking natural antibody.Nature 197, 706 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Nauciel C.: Role of CD4+ T cells and T-independent mechanisms in acquired resistance toSalmonella typhimurium infection.J. Immunol. 145, 1265–1269 (1990).

    PubMed  CAS  Google Scholar 

  • Okahashi N., Yamamoto M., VanCott J.L., Chatfield S.N., Roberts M., Bluethmann H., Hiroi T., Kiyono H., McGhee J.R.: Oral immunization of IL-4 knockout mice with recombinantSalmonella strain or cholera toxin reveals that CD4+ Th2 cells producing IL-5 and IL-10 are associated with mucosal IgA responses.Infect. Immun. 64, 1516–1525 (1996).

    PubMed  CAS  Google Scholar 

  • Pegues D.A., Miller S.I.: Salmonellosis, including typhoid fever.Curr. Opin. Infect. Dis. 7, 616–623 (1994).

    Article  Google Scholar 

  • Pie S., Truffa-Bachi P., Pla M., Nauciel C.: Th1 response inSalmonella typhimurium— infected mice with a high or low rate of bacterial clearance.Infect. Immun. 65, 4500–4514 (1997).

    Google Scholar 

  • Řeháková Z., Trebichavský I., Šplíchal I., Šinkora M., Šinkora J.: Early ontogeny of monocytes and macrophages in the pig.Physiol. Res. submitted (1998).

  • Schwager J., Schulze J.: Maturation of the mitogen responsiveness, and IL2 and IL6 production by neonatal swine leukocytes.Vet. Immunol. Immunopathol. 57, 105–119 (1997).

    Article  PubMed  CAS  Google Scholar 

  • Tlaskalová-Hogenová H., Mandel L., Dlabač V., Prokešová L., Hofman J., Kovářů F.: Pathophysiological and immunological effects of lipopolysaccharide in germfree and conventional piglets, inBacterial Endotoxin and Host Response (M.K. Agarwal, Ed.). Elsevier North Holland Biomedical Press, Amsterdam 1980.

    Google Scholar 

  • Tlaskalová-Hogenová H., Černá J., Mandel L.: Peroral immunization of germfree piglets: appearance of antibody-forming cells and antibodies of different isotypes.Scand. J. Immunol. 13, 467–472 (1981).

    Article  PubMed  Google Scholar 

  • Trávníček J., Mandel L.: Haematology of conventional and germfree miniature Minnesota piglets. II.Z. Versuchstierk. 24, 308–317 (1982).

    Google Scholar 

  • Trebichavský I., Dlabač V., Řeháková Z., Zahradníčková M., Šplíchal I.: Cellular changes and cytokine expression in the ilea of gnotobiotic piglets resulting from peroralSalmonella typhimurium challenge.Infect. Immun. 65, 5244–5249 (1997a).

    PubMed  Google Scholar 

  • Trebichavský I., Mára M., Šinkora J., Šplíchal I., Štěpánková R.: Immunomodulatory effects ofBacillus firmus.Folia Microbiol. 42, 403–408 (1997b).

    Google Scholar 

  • Yabiki T., Kashiwazaki M., Namioka S.: Quantitative analysis of three classes of immunoglobulin in serum of newborn pigs and milk of sows.Am. J. Vet. Res. 35, 1483–1489 (1974).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Trebichavský, I., Schulze, J., Dlabač, V. et al. Salmonellosis: Lessons drawn from a germ-free pig model. Folia Microbiol 43, 697–701 (1998). https://doi.org/10.1007/BF02816393

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02816393

Keywords

Navigation