Abstract
Triglycerides, oleic acid but not fatty acid-containing, nonionic detergents (Spans, Tweens) were able to stimulate the synthesis of cell-bound and soluble lipase ofYarrowia lipolytica grown in a complex medium containing citrate and urea. The optimal concentration of olive oil for induction was 0.5% (W/V). The combined effect of a high ionic strength (0.75 mol/L KCl) and of digitonin (2 mmol/L) at pH 7.6 resulted in solubilization of 80% of the cell-bound lipase and a significant activation of the enzyme. Comparison of twoY. lipolytica strains showed the effects of Mg2+ and Fe3+ concentrations in the medium on the synthesis of the enzyme to be strongly strain-dependent.
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Novotnyý, Č., Doležalová, l. Effect of lipids and detergents on the production and solubilization of extracellular lipase inYarrowia lipolytica . Folia Microbiol 38, 49–54 (1993). https://doi.org/10.1007/BF02814549
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DOI: https://doi.org/10.1007/BF02814549