Abstract
Hyperthermophilic archaea, specificallyPyrococcus spp., are the target of current efforts in developing heterologous expression systems. However, the published plasmid purification and plasmid screening protocols are long and tedious. We describe a fast, simple protocol for plasmid purification fromPyrococcus spp. developed while extracting the plasmid pGT5 fromPyrococcus abyssi cells. The protocol is modified from the procedures for commercial plasmid minipreps and is completed in about 20 min. The DNA is easily digested by restriction enzymes and can be used in sequencing reactions without additional purification.
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González, J.M., Robb, F.T. Rapid extraction of plasmid pGT5 from the hyperthermophilic archaeonPyrococcus abyssi . Mol Biotechnol 11, 221–224 (1999). https://doi.org/10.1007/BF02788680
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DOI: https://doi.org/10.1007/BF02788680