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The effect of cellular energetics on foreign protein production

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Abstract

Escherichia coli strain F-122 was used to determine if there are additional physiological effects, other than decreasing energetic efficiency accompanied by the excretion of the acetate, on foreign protein production. This organism was the host for expressing HIV582-β-galactosidase fusion protein under the control of thetrp promoter, with ampicillin resistance. By comparing parallel batch cultures with and without acetate addition, it was found that the presence of acetate in the media did not influence β-galactosidase activity. In these experiments, it appears that the low protein productivity often observed during acetate formation is the result of inefficient cell metabolism, rather than acetate acting as a specific inhibitor of protein production.

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Abbreviations

Fc :

the carbon factor

Fe :

the energy factor

Gs :

the scaling factor

HAC :

acetate concentration

Pf :

cell mass fraction of foreign protein

g/g; γs:

cell yield

Z:

fraction of carbon flux flow into the EMP pathway at time t

n:

total glucose flux

η:

normalized efficiency coefficient

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Maryland, 20742, College Park, MD

    Yun-Fei Ko, William E. Bentley & William A. Weigand

  2. Center for Agricultural Biotechnology, University of Maryland, 20742, College Park, MD

    William E. Bentley

  3. Center for Biotechnology Manufacturing, University of Maryland, 20742, College Park, MD

    Yun-Fei Ko & William A. Weigand

Authors
  1. Yun-Fei Ko
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  2. William E. Bentley
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  3. William A. Weigand
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Ko, YF., Bentley, W.E. & Weigand, W.A. The effect of cellular energetics on foreign protein production. Appl Biochem Biotechnol 50, 145–159 (1995). https://doi.org/10.1007/BF02783451

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  • DOI: https://doi.org/10.1007/BF02783451

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