Summary
The changes in the levels of leukotrienes (LTs) and prostaglandins (PGs) in the liver tissue of rats with carbon tetrachloride (CCl4-induced liver injury were studied. As a result, after the administration of CCI4, the levels of LTs increased at an early stage while the levels of PGs increased at a later stage. This suggests that LTs may have an adverse effect on liver injury induced by CC14, and that PGs may not have a direct effect on liver injury. In addition, serum GOT and GPT levels improved with the administration of AA-861, a 5-lipoxygenase inhibitor, while these levels did not change with the administration of indomethacin, a cyclooxygenase inhibitor. These results suggest that arachidonic acid metabolites may play an important role in the induction of liver cell injury.
Similar content being viewed by others
References
Williams TJ: Prostaglandin E2, prostaglandin I2 and the vascular change of inflammation. Br J Pharmacol 1979;65:517
Samuelsson B: Leukotrienes: mediators of immediate hypersensitivity reactions and inflammation. Science 1983; 220:568–575
Hammarstrom S: Leukotrienes/ Ann Rev Biochem 1983;52:355–377
Mizoguchi Y, Tsutsui H, Miyajima K, et al: The protective effect of prostaglandin E1 in an experimental massive hepatic cell necrosis model. Hepatology 1987;7:1184–1188
Mizoguchi Y, Sakagami Y, Seki S, et al: Protective effect of a leukotriene inhibitor in an experimental massive hepatic cell necrosis model. Gastroenterol Jpn 1988;23:263–267
Brattin WJ, Glende EA, Recknagel RO: Pathological mechanisms in carbon tetrachloride hepatocytotoxicity. J Free Radicals in Biology and Medicine 1985;l:27–38
Slater TF: Free-radical mechanisms in tissue injury. J Biochem 1984;222:1–15
Stachura J, Tarnawsky A, Ivey KJ, et al: Prostaglandin protection of carbon tetrachloride-induced liver cell necrosis in rat. Gastroenterology 1981;81:211–217
Rush B, Merritt MV, Kaluzny M, et al: Studies on the mechanism of the protective action of 16, 16-dimethyl PGE2 in carbon tetrachloride-induced acute hepatic injury in the rat. Prostaglandins 1986;32:439–455
Ruwart MJ, Nichols NM, Hedeen K, et al: 16,16-dimethyl PGE2 and fatty acid protect hepatocytes against CCI4-induced damage. In Vitro Cellular and Developmental Biology 1985;21:450–452
Bergmeyer HU, Scheibe P, Wahlefeld AW: Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clin Chem 1978;24:58–73
Powell WS: Rapid extraction of oxygenated metabolites of arachidonic acid from biological samples using ocetadecylsilyl silica. Prostaglandins 1980;20:947–957
Pickett WC, Douglas MB: Automated extraction and HPLC resolution of lipoxygenase and cyclooxygenase products utilizing high pressure column switching. Prostaglandins 1985;29:83–91
Nigam S: Extraction of eicosanoids from biological samples. In: Benedetto C, McDonald-Gibson RG, Nigam S, Slater TF, eds. Prostaglandins and related substances: a practical approach. IRL Press, Oxford. 1987;45–52
Robert A, Nezamis JE, Lancaster C, et al: Cytoprotection by prostaglandins in rats. Gastroenterology 1979;77:433–443
Okumura T, Saito K: Degradation of prostaglandin E2 in a primary culture of adult rat hepatocytes. J Biochem 1984;96:429–436
Tran-Thi TA, Gyufko K, Henninger H, et al: Studies on synthesis and degeneration of eicosanoids by rat hepatocytes in primary culture. Hepatology 1987;5:322–331
Rigas A, Levine L: Arachidonic acid metabolism by rat liver cells (The C-9 cell line). J Pharmacology and Experimental Therapeutics 1984;231:230–235
Spolarics Z, Tanacs B, Garzo T, et al: Prostaglandin and thromboxane synthesizing activity in isolated murine hepatocytes and nonparenchymal liver cells. Prostaglandin Leukotriene and Medicine 1984;16:379–388
Bartolini G, Meringolo C, Orlandi M, et al: Biosynthesis of prostaglandins in parenchymal and nonparenchymal rat liver cells. Biochim Biophys Acta 1978;530:325–332
Okumura T, Nakayama R, Sago T, et al: Identification of prostaglandin E metabolites from primary cultures of rat hepatocytes. Biochim Biophys Acta 1985;837:197–207
Birmelin M, Decker K: Synthesis of prostanoids and cyclic nucleotides by phagocytosing rat Kupffer cells Eur J Biochem 1984;142:219–225
Keppler D, Hagmann W, Rapp S, et al: The relation of leukotrienes to liver injury. Hepatology 1985;5:883–891
Hagmann W, Steffan AM, Kirn A, et al: Leukotrienes as mediators in frog virus 3-induced hepatitis in rats. Hepatology 1987;7:732–736
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kawada, N., Mizoguchi, Y., Kobayashi, K. et al. Arachidonic acid metabolites in carbon tetrachloride-induced liver injury. Gastroenterol Jpn 25, 363–368 (1990). https://doi.org/10.1007/BF02779452
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02779452