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An E-selectin-IgG chimera supports sialylated moiety dependent adhesion of colon carcinoma cells under fluid flow

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Abstract

To further characterize the molecular mechanisms that govern carcinoma cell adhesion to stimulated endothelium, we studied the adhesion of a human colon carcinoma cell line, KM12-L4, to an E-selectin-IgG1 chimera and interleukin (IL)-1β-stimulated human umbilical vein endothelial cells (HUVEC) underin vitro fluid flow conditions. Between 0.6 and 1.8 dynes/cm2, KM12-L4 cells attach to and roll on IL-1β-stimulated HUVEC. The adhesion is E-selectin dependent and diminished upon pretreatment of the KM12-L4 cells with neuraminidase (neuraminidase sensitive). Between 0.7 and 1.8 dynes/cm2, surfaces coated with an E-selectin-IgG1 chimera support attachment and rolling of KM12-L4 cells. The adhesion to the E-selectin-IgG1 chimera is blocked by an antibody to the lectin domain of E-selectin and is neuraminidase sensitive. Rolling KM12-L4 cells exhibit variable velocity motion over both IL-1β-stimulated HUVEC and E-selectin-IgG1 chimera-coated surfaces. Our results provide the first direct evidence that sialylated moieties are involved in the adhesion of carcinoma cells to IL-1β-stimulated endothelium under flow conditions; E-selectin-IgG1 chimeras can support cell attachment and rolling under defined flow conditions; the topology of the endothelium is not the sole cause of variable velocity motion observed in cell rolling systems.

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Author notes

  1. Douglas J. Goetz

    Present address: Vascular Research Division, Department of Pathology, Brigham and Women’s Hospital, 221 Longwood Avenue LMRC, 02115, Boston, MA, USA

  2. Daniel A. Hammer

    Present address: School of Chemical Engineering, Cornell University, 14853, Ithaca, NY, USA

Authors and Affiliations

  1. School of Chemical Engineering, Cornell University, 14853, Ithaca, NY, USA

    Douglas J. Goetz & Brian K. Brandley

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Goetz, D.J., Brandley, B.K. & Hammer, D.A. An E-selectin-IgG chimera supports sialylated moiety dependent adhesion of colon carcinoma cells under fluid flow. Ann Biomed Eng 24 (Suppl 1), 87–98 (1995). https://doi.org/10.1007/BF02770998

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