Abstract
Several samples of microbial mat obtained from soda lakes of the Kunkurskaya steppe (Chita region) abundantly populated by purple bacteria were screened for the presence of heterotrophic alkaliphiles capable of oxidizing sulfur compounds to sulfate. This capacity was found in only one pigmented strain, ALG 1, isolated on medium with acetate and thiosulfate at pH 10. The strain was found to be a strictly aerobic and obligately heterotrophic alkaliphile. Growth on medium with acetate was possible within a narrow pH range from 8.5 to 10.4. The strain formed a reddish orange carotenoid and bacteriochlorophylla. Pigments were synthesized only at high concentrations of nitrogen-containing organic compounds (peptone or yeast extract). The production of bacteriochlorophylla was maximal under microaerobic conditions in darkness. Strain ALG 1 could oxidize sulfide, thiosulfate, sulfite, and elemental sulfur to sulfate. In heterotrophically growing culture (pH 10), thiosulfate was not oxidized until the late logarithmic phase. The sulfur-oxidizing activity was maximal at the most alkaline pH values. The notable increase in the efficiency of organic carbon utilization observed in the presence of thiosulfate suggested that the bacterium was a sulfur-oxidizing lithoheterotroph. The phylogenetic analysis of the 16S rRNA gene showed strain ALG 1 to be a member of the α-3 subgroup of Proteobacteria and to constitute a distinct branch located between nonsulfur purple bacteriaRhodobacter andRhodovulum. Based on the unique phenotypic properties and the results of phylogenetic analysis, the alkaliphilic isolate ALG 1 was assigned to a new genus and speciesRoseinatronobacter thiooxidans with the type strain DSM-13087
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Horikoshi, K., Alkaliphiles-from an Industrial Point of View,FEMS Microbiol Rev., 1996, vol. 18, pp. 259–270.
Jones, B.E., Grant, W.D., Duckworth, A.W., and Owenson, G.G., Microbial Diversity of Soda Lakes,Extremophiles, 1998, vol. 2, pp. 191–200.
Zavarzin, G.A., Zhilina, T.N., and Kevbrin, V.V., The Alkaliphilic Microbial Community and Its Functional Diversity,Mikrobiologiya, 1999, vol. 68, no. 5.
Issatchenko, B.L., Chloride, Sulfate, and Soda Lakes of the Kulundinskaya Steppe and the Biogenic Processes Therein, Moscow: Akad. Nauk SSSR, 1951, pp. 143–162.
Imhoff, J.F., Sahl, H.G., Soliman, G.S.H., and Trüper, H.G., The Wadi Natrun: Chemical Composition and Microbial Mass Developments in Alkaline Brines of Eutrophic Desert Lakes,Geomicrobiol. J., 1979, vol. 1, pp. 219–234.
Tindall, B.J., Phototrophic Bacteria from Kenyan Soda Lakes,Ph.D. Thesis, Leicester Univ., 1980.
Bryantseva, I.A., Gorlenko, V.M., Kompantseva, E.I., Imhoff, J.F., Suling, J., and Mityushina, L.L.,Thiorhodospira sibirica gen. nov., sp. nov., a New Alkaliphilic Purple Sulfur Bacterium from a Siberian Soda Lake,Int. J. Syst. Bacteriol., 1999, vol. 49, pp. 697–703.
Zhilina, T.N., Zavarzin, G.A., Rainey, F.A., Pikuta, E.V., Osipov, G.A., and Kostrikina, N.A.,Desulfonatronovibrio hydrogenovorans gen. nov., sp. nov., an Alkaliphilic Sulfate-reducing Bacterium,Int. J. Syst. Bacteriol., 1997, vol. 47, pp. 144–149.
Sorokin, D.Yu., Robertson, L.A., and Kuenen, J.G., Isolation and Characterization of Alkaliphilic Chemolithotrophic, Sulphur-oxidizing Bacteria,Antonie van Leeuwenhoek, 1999 (in press).
Sorokin, D.Yu., Lysenko, A.M., and Mityushina, L.L., Isolation and Characterization of Alkaliphilic Chemoorganotrophic Bacteria Oxidizing Reduced Inorganic Sulfur Compounds to Tetrathionate,Mikrobiologiya, 1996, vol. 65, pp. 370–383.
Sorokin, D.Yu. and Kuenen, J.G., A New Facultatively Autotrophic Hydrogen-oxidizing Bacterium from Alkaline Environment,Extremophiles, 2000 (in press).
Pfennig, N. and Lippert, K.D., Uber das Vitamin B12Bedürfnis phototropher Schwefel Bacterien,Arch. Mikrobiol, 1966, vol. 55, pp. 245–256.
Sorokin, D.Yu.,Sulfitobacter pontiacus gen. nov., sp.nov.-a New Heterotrophic Bacterium from the Black Sea, Specialized on Sulfite Oxidation,Mikrobiologiya, 1995, vol. 64, pp. 354–365.
Kelly, D.P., Chambers, L.A., and Trudinger, P.A., Cyanolysis and Spectrophotometric Estimation of Trithionate in Mixture with Thiosulfate and Tetrathionate,Anal. Chem., 1969, vol. 41, pp. 898–901.
Cypionka, H. and Pfennig, N., Growth Yield ofDesulfotomaculum orientis with Hydrogen in Chemostat Culture,Arch. Microbiol., 1986, vol. 143, pp. 396–399.
Yurkov, V.V. and van Gemerden, H., Impact of Light/Dark Regime on Growth Rate, Biomass Formation and Bacteriochlorophyll Synthesis inErythromicrobium hydrolyticum, Arth. Microbiol, 1993, vol. 159, pp. 84–89.
Marmur, J., A Procedure for the Isolation of Deoxyribonucleic Acid from Microorganisms,J. Mol. Biol, 1961, vol. 3, pp. 208–218
De Ley, J., Cattoir, H., and Reynaerts, A., The Quantitative Measurements of DNA Hybridization from Renaturation Rates,Eur. J. Biochem., 1970, vol. 12, pp. 133–140.
Yurkov, V.V. and Beaty, T., Aerobic Anoxygenic Phototrophic Bacteria,Microbiol. Mol. Biol. Rev., 1998, vol. 62, pp. 695–724.
Sorokin, D.Yu., The Effect of Thiosulfate on the Growth of Continuous Cultures of Black Sea Isolates of Heterotrophic Bacteria Oxidizing Sulfur Compounds to Sulfate,Mikrobiologiya, 1994, vol. 63, pp. 255–259.
Sorokin, D.Yu. and Lysenko, A.M., Characterization of Black Sea Isolates of Heterotrophic Bacteria Oxidizing Sulfur Compounds to Sulfate,Mikrobiologiya, 1993, vol. 62, pp. 1018–1031.
Yurkov, V.V, Krasil’nikova, E.N., and Gorlenko, V.M., Metabolism of Thiosulfate in Aerobic Bacteriochlorophyll a-containing Bacteria,Mikrobiologiya, 1994, vol. 63, pp. 181–188.
Woese, C.R., Stackebrandt, E., Weisburg, W.G., Paster, B.J., Madigan, M.T., Fowler, V.J., Hahn, C.M., Blanz, P., Gupta, R., Nelson, K., and Fox, G.E., The Phylogeny of Purple Bacteria: The Alpha Subdivision,Syst. Appl. Microbiol, 1984, vol. 5, pp. 315–326.
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Sorokin, D.Y., Tourova, T.P., Kuznetsov, B.B. et al. Roseinatronobacter thiooxidans gen. nov., sp. nov., a new alkaliphilic aerobic bacteriochlorophylla—containing bacterium isolated from a soda lake. Microbiology 69, 75–82 (2000). https://doi.org/10.1007/BF02757261
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DOI: https://doi.org/10.1007/BF02757261