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Tissue-specific expression and regulation by 1,25(OH)2D3 of chick protein kinase inhibitor (PKI) mRNA

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Abstract

The heat-stable protein kinase inhibitor (PKI) protein is a specific and potent competitive inhibitor of the catalytic subunit of cAMP-dependent protein kinase (PKA). Previously, it has been shown that vitamin D status affects chick kidney PKI activity: a 5- to 10-fold increase in PKI activity was observed in kidneys of chronically vitamin D-deficient chicks and treatment with 1,25-dihydroxyvitamin D3 (1,25[OH]2D3) in cultured kidney cells resulted in a 95% decrease in PKI activity. The authors have recently cloned the cDNA for chick kidney PKI and have used the coding sequence to study the regulation of PKI mRNA. Northern analysis showed the expression of two PKI messages, which are 2.7 and 3.3 kb in size. These mRNAs are expressed in brain, muscle, testis, and kidney, but not in pancreas, liver, or intestine. PKI mRNA steady-state levels are downregulated by 47% in kidneys from vitamin D-replete chicks as compared to vitamin D-deficient chicks. PKI mRNA levels in brain, muscle, and testis are not affected by vitamin D status. Treatment of primary chick kidney cultures treated with 10−7 M, 1,25(OH)2D3 for 24 h resulted in a 20–30% decrease in PKI mRNA. 1,25(OH)2D3 treatment does not affect the stability of PKI mRNA as determined by treatment of cell cultures with actinomycin D. This study shows that 1,25(OH)2D3 directly and tissue-specifically downregulates PKI mRNA in the chick kidney.

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References

  1. Posner, J. B., Stern, R., and Krebs, E. G. (1965).J. Biol. Chem. 240, 982–985.

    PubMed  CAS  Google Scholar 

  2. Ashby, C. D. and Walsh, D. A. (1972).J. Biol. Chem. 247, 6637–6642.

    PubMed  CAS  Google Scholar 

  3. Wen, W., Harootunian, A. T., Adams, S. R., Feramisco, J., Tsien, R. Y., Meinkoth, J. L., and Taylor, S. S. (1994).J. Biol. Chem. 269, 32,214–32,220.

    CAS  Google Scholar 

  4. Wen, W., Meinkoth, J. L., Tsien, R. Y., and Taylor, S. S. (1995).Cell 82, 463–473.

    Article  PubMed  CAS  Google Scholar 

  5. Szmigielski, A., Guidotti, A., and Costa, E. (1977).J. Biol. Chem. 252, 3848–3853.

    PubMed  CAS  Google Scholar 

  6. Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E. H., and Krebs, E. G. (1971).J. Biol. Chem. 246, 1977–1985.

    PubMed  CAS  Google Scholar 

  7. Skala, J. P., Drummond, G. I., and Hahn, P. (1974).Biochem. J. 138, 195–199.

    PubMed  CAS  Google Scholar 

  8. Henry, H. L., Al-Abdaly, F. A., and Noland, T. A. (1983).Comp. Biochem. Physiol. 74B, 715–718.

    CAS  Google Scholar 

  9. Ashby, C. D. and Walsh, D. A. (1973).J. Biol. Chem. 248, 1255–1261.

    PubMed  CAS  Google Scholar 

  10. Walsh, D. A. and Ashby, C. D. (1973).Recent. Prod. Hormone Res. 29, 329–359.

    CAS  Google Scholar 

  11. Kuo, J. F. (1975).Biochem. Biophys. Res. Commun. 65, 1214–1220.

    Article  PubMed  CAS  Google Scholar 

  12. Costa, M. (1977).Biochem. Biophys. Res. Commun. 78, 1311–1218

    Article  PubMed  CAS  Google Scholar 

  13. Tash, J. S., Welsh, M. J., and Means, A. R. (1981).Endocrinology 108, 427–434.

    PubMed  CAS  Google Scholar 

  14. Rudack-Garcia, D. and Henry, H. L. (1981).J. Biol. Chem. 256, 10,781–10,785.

    CAS  Google Scholar 

  15. Al-Abdaly, F. A. and Henry, H. L. (1989).Endocrinology 124, 2901–2906.

    PubMed  CAS  Google Scholar 

  16. Henry, H. L., Cunningham, N. C., and Noland, T. A. (1983).Endocrinology 113, 1942–1949.

    Article  PubMed  CAS  Google Scholar 

  17. Harrison, H. C., Harrison, H. E., and Park E. A. (1958).Am. J. Physiol. 192, 432–436.

    PubMed  CAS  Google Scholar 

  18. Marchetto, G. S. and Henry, H. L. (1995).Gene 158, 303,304.

    Article  PubMed  CAS  Google Scholar 

  19. Van Patten, S. M., Howard, P., Walsh, D. A., and Maurer, R. A. (1992).Mol. Endo. 6, 2114–2122.

    Article  Google Scholar 

  20. Walters, M. R. (1992).Endocr. Rev. 13, 719–764.

    Article  PubMed  CAS  Google Scholar 

  21. Okazaki, T., Igarashi, T., and Kronenberg, H. M. (1988).J. Biol. Chem. 263 2203–2208.

    PubMed  CAS  Google Scholar 

  22. Naveh-Many, T. and Silver, J. (1988).J. Clin. Invest. 81, 270–273.

    PubMed  CAS  Google Scholar 

  23. Lichtler, A., Stover, M. L., Angilly, J., Kream, B., and Rowe, D. W. (1989).J. Biol. Chem. 264, 3072–3077.

    PubMed  CAS  Google Scholar 

  24. Simpson, R. U., Hsu, T., Begley, D. A., Mitchell, B. S., and Alizadeh, B. N. (1987).J. Biol. Chem. 262, 4104–4108.

    PubMed  CAS  Google Scholar 

  25. Norman, A. W., Midgett, R. J., Myrtle, J. F., and Nowicki, H. G. (1971).Biochem. Biophys. Res. Commun. 42, 1082–1087.

    Article  PubMed  CAS  Google Scholar 

  26. Sanger, F., Nicklen, S., and Coulson, A. R. (1977).Proc. Natl. Acad. Sci. USA 74, 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  27. Chomczynski, P. and Sacchi, N. (1987).Anal. Biochem. 162, 156–159.

    Article  PubMed  CAS  Google Scholar 

  28. Puissant, C. and Houdebine, L. M. (1990).Biotechniques 8, 148,149.

    Article  PubMed  CAS  Google Scholar 

  29. Lehrach, H., Diamond, D., Wozney, J. M., and Boedtker, H. (1977).Biochemistry 16, 4743–4751.

    Article  PubMed  CAS  Google Scholar 

  30. Henry, H. L. (1979).J. Biol. Chem. 254, 2722–2729.

    PubMed  CAS  Google Scholar 

  31. Chou, S.-Y., Hannah, S., Lowe, K. E., Norman, A. W., and Henry, H. L. (1995).Endocrinology 136, 5520–5526.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina, Chapel Hill, NC

    Gail S. Marchetto

  2. Department of Biochemistry, University of California, 92521, Riverside, CA

    Helen L. Henry

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  1. Gail S. Marchetto
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  2. Helen L. Henry
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Correspondence to Helen L. Henry.

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Marchetto, G.S., Henry, H.L. Tissue-specific expression and regulation by 1,25(OH)2D3 of chick protein kinase inhibitor (PKI) mRNA. Endocr 6, 5–10 (1997). https://doi.org/10.1007/BF02738795

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  • DOI: https://doi.org/10.1007/BF02738795

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